EFFECT OF NITROGEN DEFICIENCY ON ION-EXCHANGE PROPERTIES OF WHEAT ROOT CELL WALL POLYMERS
Abstract
Ion-exchange properties of root cell walls of Triticum aestivum L. plants grown in hydroponic nutrient medium with nitrate or without nitrogen source were investigated. The root cell walls contained four types of ion-exchange groups irrespective of the nitrogen nutrition: primary amino groups (рКа < 3) of structural cell wall proteins, carboxyl groups of polygalacturonic acid (рКа ~4.7) of pectin, carboxyl groups of hydroxycinnamic acids (рКа ~7.3), and phenolic OH-groups of lignin (рКа ~10.2). Content of ion-exchange groups, the cell wall dry weight to root dry weight ratio, and the swelling coefficient of the cell walls were influenced by the presence of nitrate in the medium. The root cell walls of N-deficient plants had 2.4 times higher content of phenolic OH-groups, 1.24 times higher cell wall dry weight to root dry weight ratio, and 10% lower cell wall swelling coefficient, than nitrate-supplied plants. The obtained results indicate that nitrogen deficiency causes an increase in cell wall thickness, which probably is a result of increased lignin content.
About the Authors
N. R. Meychik
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Plant Physiology, School of Biology
Leninskiye gory 1-12, Moscow, 119234, Russia
Yu. I. Nikolaeva
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Plant Physiology, School of Biology
Leninskiye gory 1-12, Moscow, 119234, Russia
M. A. Kushunina
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Plant Physiology, School of Biology
Leninskiye gory 1-12, Moscow, 119234, Russia
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Review
For citations:
Meychik N.R., Nikolaeva Yu.I., Kushunina M.A. EFFECT OF NITROGEN DEFICIENCY ON ION-EXCHANGE PROPERTIES OF WHEAT ROOT CELL WALL POLYMERS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(2):87-91. (In Russ.)
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