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BIOTECHNOLOGICAL POTENTIAL ASSESSMENT AND TAXONOMIC STATUS REFINEMENT OF MICROALGAE OF THE GENUS PARIETOCHLORIS (TREBOUXIOPHYCEAE) IN THE CALU-COLLECTION

Abstract

We have carried out morphological, biochemical, as well as molecular and genetic studies of green microalgae from the Collection of cyanobacteria, algae and algal parasites of Saint-Petersburg State University (CALU), presumably belonging to the genus Parietochloris, to assess their biotechnological potential and to refine their phylogenetic position. It was found that the investigated strains have a close relationship to two genera from different classes – Lobosphaera (Trebouxiaceae) and Deasonia (Actinochloridaceae). They can be of interest as producers of valuable polyunsaturated fatty acids, especially arachidonic, linoleic, α-linolenic.

About the Authors

K. A. Shibzukhova
Lomonosov Moscow State University
Russian Federation

Department of Bioengineering, School of Biology

Leninskiye gory 1–12, Moscow, 119234, Russia



O. V. Gavrilova
Saint Petersburg State University
Russian Federation

Department of Microbiology, School of Biology

Universitetskaya nab. 7–9, Saint-Petersburg, 199034, Russia



O. B. Chivkunova
Lomonosov Moscow State University
Russian Federation

Department of Bioengineering, School of Biology

Leninskiye gory 1–12, Moscow, 119234, Russia



R. A. Sidorov
Institute of Plant Physiology, Russian Academy of Science
Russian Federation
Botanicheskaya ul. 35, Moscow, 127276, Russia


A. E. Solovchenko
Lomonosov Moscow State University
Russian Federation

Department of Bioengineering, School of Biology

Leninskiye gory 1–12, Moscow, 119234, Russia



E. S. Lobakova
Lomonosov Moscow State University
Russian Federation

Department of Bioengineering, School of Biology

Leninskiye gory 1–12, Moscow, 119234, Russia



References

1. Weber P.C., Fischer S., von Schaky C., Lorenz R., Strasser T. Dietary omega-3 polyunsaturated fatty acids and eicosanoids formation in man // Health effects of polyunsaturated fatty acid in seafood’s / Eds. A.P. Simopoulos, R.R. Kifer, and R.E. Martin. Orlando: Academic press, 1986. P. 227–238.

2. Solovchenko A.E., Khozin-Goldberg I., Didi-Cohen S., Cohen Z., Merzlyak M.N. Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa // J. Appl. Phycol. 2008. Vol. 20. N 3. P. 245–251.

3. Dumancas G.G., Murdianti B.S., Lucas E.A. Arachidonic acid. Dietary sources and general functions. N.Y.: Nova Science Publishers, 2013. 255 pp.

4. Громов Б.В., Титова Н.Н. Коллекция культур водорослей лаборатории микробиологии Биологического института Ленинградского университета (CALU) // Культивирование коллекционных штаммов водорослей. Межвуз. сб. / Под ред. Б.В. Громова. Л: Из-во Ленингр. ун-та, 1983. С. 3–27.

5. Solovchenko A., Khozin Goldberg I., Recht L., Boussiba S. Stress induced changes in optical properties, pigment and fatty acid content of Nannochloropsis sp.: Implications for non- destructive assay of total fatty acids // Mar. Biotechnol. 2011. Vol. 13. N 3. P. 527–535.

6. Ветчинникова Л.В., Серебрякова О.С., Ильинова М.К. Динамика содержания липидов и жирнокислотного состава отдельных фракций в женских сережках березы повислой (Betula pendula Roth) // Труды Карельского научного центра РАН. 2013. № 3. С. 74–81.

7. White T.J., Bruns T.D, Lee S.B., Taylor J.W. Amplification and direct sequencing of fungal ribosomal RNA for phylogenetics // PCR protocols: a guide to methods and applications. A guide to methods and applications / Eds. M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White. N.Y.: Academic Press, 1990. P. 315–322.

8. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs // Nucleic Acids Res. 1997. Vol. 25. N 17. P. 3389–3402.

9. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees // Mol. Biol. Evol. 1987. Vol. 4. N 4. P. 406–425.

10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap // Evolution. 1985. Vol. 39. N 4. P. 783–791.

11. Gorelova O.A., Kosevich I.A., Baulina O.I., Fedorenko T.A., Torshkhoeva A.Z., Lobakova E.S. Associations between the White Sea invertebrates and oxygen-evolving phototrophic microorganisms // Moscow Univ. Biol. Sci. Bull. 2009. Vol. 64. N 1. P. 16–22.


Review

For citations:


Shibzukhova K.A., Gavrilova O.V., Chivkunova O.B., Sidorov R.A., Solovchenko A.E., Lobakova E.S. BIOTECHNOLOGICAL POTENTIAL ASSESSMENT AND TAXONOMIC STATUS REFINEMENT OF MICROALGAE OF THE GENUS PARIETOCHLORIS (TREBOUXIOPHYCEAE) IN THE CALU-COLLECTION. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(3):158-163. (In Russ.)

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ISSN 0137-0952 (Print)