INFLUENCE OF SODIUM AND POTASSIUM IONS ON CONFORMATION OF LINKER PARTS OF NUCLEOSOMES
Abstract
The structure of chromatin and DNA accessibility to proteins depend on the structure of DNA “linkers” entering and exiting the nucleosome. Since DNA is negatively charged, conformation of the DNA linkers, in turn, is likely affected by ionic microenvironment. Here method of fluorescence microscopy of single complexes was used to study the effect of Na+ and K+ ions on the structure of the DNA linkers. It was revealed that nucleosomes adopt one of two conformational states, and occupancy of these states is considerably changed after substitution of K+ ions by Na+. These changes are likely caused by different interaction of Na+ and K+ ions with DNA in the regions of entry and exit of DNA from the nucleosome. Cation- dependent changes in conformation of the DNA linkers can affect topological barriers in nucleosomes, structure of polynucleosome chromatin and interactions of DNA with different protein factors.
Keywords
chromatin,
nucleosome,
linkers,
sodium ions,
potassium ions,
fluorescence,
microscopy,
single molecule
About the Authors
A. V. Lyubitelev
Lomonosov Moscow State University
Russian Federation
Russian Federation
Bioengineering Department, School of Biology
Leninskiye Gory 1–12, Moscow, 119234, Russia
V. M. Studitsky
Lomonosov Moscow State University
Fox Chase Cancer Center
Russian Federation
Russian Federation
Bioengineering Department, School of Biology
Leninskiye Gory 1–12, Moscow, 119234, Russia
333 Cottman avenue, Philadelphia, Pennsylvania 19111, USA
A. V. Feofanov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Bioengineering Department, School of Biology
Leninskiye Gory 1–12, Moscow, 119234, Russia
M. P. Kirpichnikov
Lomonosov Moscow State University
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation
Russian Federation
Bioengineering Department, School of Biology
Leninskiye Gory 1–12, Moscow, 119234, Russia
Miklukho-Maklaya ul. 16/10, 117997, Moscow, Russia
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Review
For citations:
Lyubitelev A.V., Studitsky V.M., Feofanov A.V., Kirpichnikov M.P. INFLUENCE OF SODIUM AND POTASSIUM IONS ON CONFORMATION OF LINKER PARTS OF NUCLEOSOMES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(3):169-173. (In Russ.)
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