THE EFFECT OF MEDIUM WAVE ULTRAVIOLET RADIATION ON THE CONTENT AND SPECTRUM OF POLYAMINES IN LEAVES AND ROOTS OF WILD-GROWING PLANTS

This work is a continuation of a series of studies on the short-term effects of UV-B radiation (12.5 kJ/m2) on plants with particular attention paid to the rapid response of the antioxidant system (measurement 24 hrs after irradiation). Free and conjugated forms of putrescine polyamines (putrescine, spermine, spermidine) and cadaverine are known to be part of the antioxidant system. In the plants Thellungiella salsuginea, Salvia officinalis L, Plantago major L., and Geum urbanum L. grown in aquatic culture under phytotron conditions, the content of free and conjugated forms of polyamins was determined 24 hrs after the treatment with UV-B. The obtained data confirm the existing hypothesis about the major role of putrescine in the protective response of plants to UV-B irradiation. An increase in the content of this polyamine was observed in the leaves of three of the four plants studied. And it was the changes in its content that determined the total increase in the level of free polyamines. It was not possible to reveal a general trend in the level of conjugated forms of spermine, spermidine and cadaverine, and only for putrescine conjugates there was a clear increase. The data obtained allow to conclude that the relative contribution of specific forms of polyamines to the protective response is determined primarily by the species of the investigated plants. It is likely that conjugated forms of polyamines can serve as a reserve for rapid recovery of the required level of endogenous free forms of polyamines.

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