SELECTION OF SPECIES FOR THE LABORATORY ALGAL COMMUNITY BY THEIR HYDROBIOLOGICAL AND BIOPHYSICAL CHARACTERISTICS
Abstract
Phytoplankton communities can serve as bioindicators of water system condition. Model of the natural ecosystem can enable multivariate experiments on the effect of physical and chemical factors on biophysical and hydrobiological characteristics of phytoplankton. Creating of such a model requires selecting appropriate species of microalgae. This study has allowed for selecting six types from those available in museum to create a model algal community. We found that similar conditions are required for their optimal growth (light, temperature, medium nutrients supply). As a base medium it is proposed to use a medium with low nitrogen content. Under these conditions, the cells function in a proper way and the cultures show satisfactory growth, while the duration of reaching the stationary stage of growth (10–15 days) allows to have more experiments for a limited time. Cells of selected species have morphological differences that are sufficient for the automated identification within the polyculture. We have obtained the geometric characteristics of cells for computer counting of each community species on microphotographs.
About the Authors
P. V. Fursova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia
E. N. Voronova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia
A. P. Levich
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia
D. V. Risnik
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia
S. I. Pogosyan
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia
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Review
For citations:
Fursova P.V., Voronova E.N., Levich A.P., Risnik D.V., Pogosyan S.I. SELECTION OF SPECIES FOR THE LABORATORY ALGAL COMMUNITY BY THEIR HYDROBIOLOGICAL AND BIOPHYSICAL CHARACTERISTICS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(4):215-221. (In Russ.)
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