SECRETION OF EXTRACELLULAR PROTEINASES, ACTIVE AGAINST FIBRILLARY PROTEINS, BY MICROMYCETES
Abstract
Micromycetes Aspergillus ustus 1 and Tolypocladium inflatum k1 were shown to produce proteolytic enzymes with high collagenolytic, fibrinolytic and elastolytic activity. The activity of the proteinases against fibrillar proteins was determined by the cleavage of azocollagen: collagenolytic activity was 122.6•10–3 EAzc/ml (EAzc – the amount of azocollagen cleaved in 1 min in micrograms) for proteinases produced by A. ustus 1 and 69.7•10–3 EAzc/ml for proteinases produced by T. inflatum k1. The maximum activity values were observed at submerged cultivation of A. ustus 1 during 4 days, and T. inflatum k1 during 5 days. It has been shown that the maximum of collagenolytic and general proteolytic activity during the cultivation of A. ustus 1 are time-separated, unlike T. inflatum k1. This fact, presumably, can simplify the procedure for obtaining of active proteinases.
About the Authors
E. A. Popova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, Moscow, 119234, Russia
D. M. Bednenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, Moscow, 119234, Russia
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, Moscow, 119234, Russia
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, Moscow, 119234, Russia
I. B. Kotova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, Moscow, 119234, Russia
N. S. Egorov
Lomonosov Moscow State University
Russian Federation
Russian Federation
International Biotechnology Center School of Biology
Leninskiye Gory 1–12, Moscow, 119234, Russia
References
1. Wanderley M.C. de A., Neto J.M.W.D., Filho J.L. de L., Lima C. de A., Teixeira J.A.C., Porto A.L.F. Collagenolytic enzymes produced by fungi: a systematic review // Braz. J. Microbiol. 2017. Vol. 48. N 1. P. 13–24.
2. Kotb E. The biotechnological potential of fibrinolytic enzymes in the dissolution of endogenous blood thrombi // Biotechnol. Prog. 2014. Vol. 30. N 3. P. 656–672.
3. Sharkova T.S., Kurakov A.V., Osmolovskiy A.A., Matveeva E.O., Kreyer V.G., Baranova N.A., Egorov N.S. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes // Microbiology. 2015. Vol. 84. N 3. P. 359–364.
4. Rudenskaya G.N. Brachyurins, serine collagenolytic enzymes from crabs // Russ. J. Bioorg. Chem. 2003. Vol. 29. N 2. P. 101–111.
5. Watanabe K. Collagenolytic proteases from bacteria // Appl. Microbiol. Biotechnol. 2004. Vol. 63. N 5. P. 520–526.
6. Hasan S., Ahmad A., Purwar A., Khan N., Kundan R., Gupta G. Enzymes in the entomopathogenic fungus Verticillium lecanii // Bioinformation. 2013. Vol. 9. N 5. P. 238–242.
7. Yike I. Fungal proteases and their pathophysiological effects // Mycopathologia. 2011. Vol. 171. N 5. P. 299–323.
8. Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.
9. Gupta P., Samant K. Sahu A. Isolation of cellulosedegrading bacteria and determination of their cellullytic potential // Int. J. Microbiol. 2012. Vol. 22. Article ID 578925.
10. Behera B.C., Parida S., Dutta S.K., Thatoi N.H. Isolation and identification of cellulose degrading bacteria from angrove soil of Mahanadi river delta and their cellulose production ability // Am. J. Microbiol. Res. 2014. Vol. 2. N 1. P. 41–46.
11. Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1 // Moscow Univ. Biol. Sci. Bull. 2016. Vol. 71. N 1. P. 62–66.
12. Chavira R. Jr., Burnett T.J., Hageman J.N. Assaying proteinase with Azocoll // Anal. Biochem. 1984. Vol. 136. N 2. P. 446–450.
13. El-Aassar S.A., El-Badry H.M., Abdel-Fattah A.F. The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9 // Appl. Microbiol. Biotechnol. 1990. Vol. 33. N 1. P. 26–30.
14. Osmolovskiy A.A., Kurakov A.V., Kreyer V.G., Baranova N.A., Egorov N.S. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system // Moscow Univ. Biol. Sci. Bull. 2017. Vol. 72. N 1. P. 20–24.
15. Lima C.A., Viana Marques D.A., Neto B.B., Lima Filho J.L., Carneiro-da-Cunha M.G., Porto A.L.F. Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622 // Biotechnol. Prog. 2011. Vol. 27. N 5. P. 1470–1477.
16. Егоров Н.С., Ландау Н.С., Буяк Л.И., Крейер В.Г. Гидролитическая система нокардиоформной бактерии Nocardia minima в процессе ее роста, развития и дифференциации // Микробиология. 1991. Т. 60. № 4. С. 637–643.
Review
For citations:
Popova E.A., Bednenko D.M., Osmolovskiy A.A., Kreyer V.G., Kotova I.B., Egorov N.S. SECRETION OF EXTRACELLULAR PROTEINASES, ACTIVE AGAINST FIBRILLARY PROTEINS, BY MICROMYCETES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(4):241-245. (In Russ.)
Views:
341
Email this article 