DIVERSITY OF POLYKETIDE SYNTHASE GENES IN THE GENOMES OF HETEROTROPHIC MICROORGANISMS ISOLATED FROM EPILITHIC BIOFILMS IN LAKE BAIKAL
Abstract
Many bacterial secondary metabolites including pharmacologically promising compounds are synthesized by polyketide synthases (PKS) enzyme complexes. Nucleotide sequences of genes encoding 16S rRNA and PKS of heterotrophic bacterial strains isolated from epilithic biofilms in the littoral zone of Lake Baikal were determined. Based on molecular phylogenetic analysis of 16S rRNA genes, we identified six heterotrophic strains: Serratia fonticola 1А and 10А, Pseudomonas umsongensis K10-2 and K10-3, Rheinheimera tilapiae K18 and Flavobacterium sp. 43-09. Sequencing of cloned amplification products for PKS gene cluster revealed 33 sequences. Genes involved in biosynthesis of antibiotics (difficidine, erythromycin, curacin, mixalamide, corallopyronin, and myxothiazol) and cytostatics (romidepsin, spiruchostatin, and disorazol) were determined among related sequences. The low homology (50–83%) of amino acid sequences of PKS in Baikal bacteria with sequences in GenBank attests to potential capability of strains to produce new, yet not studied polyketide substances. The results obtained show that the strains under investigation may be of practical interest for biotechnological application.
About the Authors
E. V. Sukhanova
Limnological Institute, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
E. A. Zimens
Limnological Institute, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
V. V. Parfenova
Limnological Institute, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
O. I. Belykh
Limnological Institute, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
Russian Federation
Ulan-Batorskaya ul. 3, Irkutsk, 664033, Russia
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Review
For citations:
Sukhanova E.V., Zimens E.A., Parfenova V.V., Belykh O.I. DIVERSITY OF POLYKETIDE SYNTHASE GENES IN THE GENOMES OF HETEROTROPHIC MICROORGANISMS ISOLATED FROM EPILITHIC BIOFILMS IN LAKE BAIKAL. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(4):246-252. (In Russ.)
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