ROLE OF NHP6 PROTEIN IN TRANSCRIPTION THROUGH A NUCLEOSOME IN VITRO
Abstract
Nhp6 is a small yeast protein, which binds DNA nonspecifically. It was shown that Nhp6 is a part of several protein complexes (including FACT complex) and is presented on many yeast promoters and transcribed regions of genes in vivo. It also participates in the process of destabilizing the structure of nucleosomes in vitro. In our laboratory, we studied FACT complex and showed its role in the transcription of eukaryotic RNA-polymerase 2 in vitro, but the role of the Nhp6 protein in transcription has not been studied previously. In this paper, we describe the effect of Nhp6 protein on transcription through a nucleosome by eukaryotic RNA-polymerase 2 and show that Nhp6 protein increases the transcription efficiency at several positions on the nucleosomal DNA, primarily the transcription at the positions +(11–17) in the nucleosome. We proposed a model of the action of Nhp6 during transcription through chromatin, suggesting the stabilization of DNA transiently uncoiled from the octamer during this process.
About the Authors
F. K. Hsieh
Harvard Medical School
United States
United States
Department of Molecular Biology
25 Shattuck St., Boston, MA 02114, USA
A. L. Kozlova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, School of Biology
1–12 Leninskiye Gory, Moscow, 119234, Russia
N. S. Gerasimova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, School of Biology
1–12 Leninskiye Gory, Moscow, 119234, Russia
E. Kotova
Cancer Epigenetics Program, Fox Chase Cancer Center
United States
United States
Cottman Avenue 333, Philadelphia, PA 19111, USA
T. Formosa
University of Utah, School of Medicine
United States
30 N 1900 E, Salt Lake City, Utah, UT 84132, USA
United States
30 N 1900 E, Salt Lake City, Utah, UT 84132, USA
V. M. Studitsky
Lomonosov Moscow State University
Cancer Epigenetics Program, Fox Chase Cancer Center
Russian Federation
Russian Federation
Department of Bioengineering, School of Biology
1–12 Leninskiye Gory, Moscow, 119234, Russia
Cottman Avenue 333, Philadelphia, PA 19111, USA
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Review
For citations:
Hsieh F.K., Kozlova A.L., Gerasimova N.S., Kotova E., Formosa T., Studitsky V.M. ROLE OF NHP6 PROTEIN IN TRANSCRIPTION THROUGH A NUCLEOSOME IN VITRO. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(4):253-257. (In Russ.)
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