BINASE INDUCED CHANGES OF TUMOR CELL MEMBRANES
Abstract
Exogenous ribonuclease of bacilli can selectively induce apoptosis of malignant cells. The analysis of the ability of Bacillus pumilus ribonuclease – binase – to induce processes, which lead to a dynamic disruption of the integrity of A549 human adenocarcinoma cell membranes, was performed. The influence of different enzyme concentrations on the state of the cytoplasmic membrane of cells and mitochondrial membranes was characterized. Using the methods of flow cytometry and fluorescence microscopy, it has been established that binase leads to normal functioning disruption of both types of membranes, with mitochondrial membranes affected first.
The study allowed to identify and visualize the effects of binase on the membrane structures of target cells and to confirm that bacterial RNase induces apoptosis of target cells mainly through the “internal” (mitochondrial) pathway.
About the Authors
P. V. Zelenikhin
Kazan Federal University
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
A. S. Gorbunova
Kazan Federal University
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
K. Beuerlein
Rudolf Buchheim Institute of Pharmacology, Justus-Liebig-University
Germany
Schubertstrasse 81, Giessen, 35392, Germany
Germany
Schubertstrasse 81, Giessen, 35392, Germany
A. V. Makeeva
Kazan Federal University
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
O. N. Ilinskaya
Kazan Federal University
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
Russian Federation
Kremlyovskaya st. 18, Kazan, 420008, Russia
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Review
For citations:
Zelenikhin P.V., Gorbunova A.S., Beuerlein K., Makeeva A.V., Ilinskaya O.N. BINASE INDUCED CHANGES OF TUMOR CELL MEMBRANES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(1):10-15. (In Russ.)
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