DEVELOPMENT OF THE ST/J/V GENOME SPECIFIC MOLECULAR MARKER BASED ON 5S RDNA POLYMORPHISM IN THINOPYRUM BESSARABICUM, PSEUDOROEGNERIA SPICATA AND DASYPYRUM VILLOSUM
Abstract
Non-transcribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp for V, 171 bp for St and 172 bp for J genome. The fragment of St genome is characterized by SmiM I restriction site that enables its differentiation from J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids.
About the Authors
O. S. Alexandrov
Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Timiryazevskaya st. 49, Moscow, 127550, Russia
M. G. Divashuk
Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy;
All-Russia Research Institute of Agricultural Biotechnology
Russian Federation
Timiryazevskaya st. 49, Moscow, 127550, Russia;
Timiryazevskaya st. 42, Moscow, 12755, Russia
Russian Federation
Timiryazevskaya st. 49, Moscow, 127550, Russia;
Timiryazevskaya st. 42, Moscow, 12755, Russia
G. I. Karlov
Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy;
All-Russia Research Institute of Agricultural Biotechnology
Russian Federation
Timiryazevskaya st. 49, Moscow, 127550, Russia;
Timiryazevskaya st. 42, Moscow, 12755, Russia
Russian Federation
Timiryazevskaya st. 49, Moscow, 127550, Russia;
Timiryazevskaya st. 42, Moscow, 12755, Russia
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Review
For citations:
Alexandrov O.S., Divashuk M.G., Karlov G.I. DEVELOPMENT OF THE ST/J/V GENOME SPECIFIC MOLECULAR MARKER BASED ON 5S RDNA POLYMORPHISM IN THINOPYRUM BESSARABICUM, PSEUDOROEGNERIA SPICATA AND DASYPYRUM VILLOSUM. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(1):22-28. (In Russ.)
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