PHOTOCURABLE HYDROGELS CONTAINING METHACRYLATED GELATIN AND SPIDROIN OR FIBROIN
Abstract
Photocurable hydrogels were fabricated from methacrylated gelatin and silk proteins, including recombinant analogue of spidroin from Nephila clavipes spider web and fibroin from the cocoons of the silkworm Bombyx mori. These polymers have high applicability in tissue engineering due to their biocompatibility and biodegradability. Hydrogels were fabricated using two different methods that allowed us to obtain either large-sized products or microstructures of certain shape.
For the production of extensive hydrogels, samples were photopolymerized in the UV light within ten minutes. As a result samples of hydrogels were obtained as disks with a diameter of 13 mm.
Scanning electron microscopy confirmed their porous structure. Microstructures were formed on coverslips using confocal microscope Eclipse Ti-E with 405 nm laser. This approach gives us an opportunity to control the topographic features of the obtained substrates and is applicable for creating micropatterns for studying the interaction of cells with a substrate.
About the Authors
I. V. Bessonov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
M. S. Kotliarova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
M. N. Kopitsyna
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
A. V. Fedulov
M.F. Vladimirsky Moscow Regional Scientific Research Clinical Institute
Russian Federation
Russian Federation
Shepkina st. 61/2–1, Moscow, 129110, Russia;
A. M. Moysenovich
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
A. Yu. Arkhipova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Laboratory of Confocal Microscopy, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia;
V. G. Bogush
State Research Institute for Genetics and Selection of Industrial Microorganisms of National Research Center “Kurchatov Institute”
Russian Federation
1-st Dorozhniy pr. 1, Moscow, 117545, Russia
Russian Federation
1-st Dorozhniy pr. 1, Moscow, 117545, Russia
D. V. Bagrov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
A. A. Ramonova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Laboratory of Confocal Microscopy, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia;
A. E. Mashkov
M.F. Vladimirsky Moscow Regional Scientific Research Clinical Institute
Russian Federation
Russian Federation
Shepkina st. 61/2–1, Moscow, 129110, Russia;
K. V. Shaitan
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering
Leninskiye gory 1–12, Moscow, 119234, Russia;
M. M. Moisenovich
Lomonosov Moscow State University
Russian Federation
Russian Federation
Laboratory of Confocal Microscopy, School of Biology
Leninskiye gory 1–12, Moscow, 119234, Russia;
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Review
For citations:
Bessonov I.V., Kotliarova M.S., Kopitsyna M.N., Fedulov A.V., Moysenovich A.M., Arkhipova A.Yu., Bogush V.G., Bagrov D.V., Ramonova A.A., Mashkov A.E., Shaitan K.V., Moisenovich M.M. PHOTOCURABLE HYDROGELS CONTAINING METHACRYLATED GELATIN AND SPIDROIN OR FIBROIN. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(1):29-33. (In Russ.)
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