SECRETION OF PROTEINASES WITH FIBRINOLYTIC ACTIVITY BY MICROMYCETES OF THE GENUS ASPERGILLUS
Abstract
Proteolytic activity of extracellular enzymes of 11 strains of different Aspergillus species was studied. Comparison of the enzymatic indices of strains grown on agar medium with casein and fibrin allowed us to select the strain A. terreus 2 as a promising producer of fibrinolytic proteases.
It was found that A. terreus 2 proteases show maximum activity at pH 8.0. The highest values of fibrinolytic and total proteolytic activities expressed in UTyr (amount of micromoles of tyrosine released from fibrin or casein for 1 min) were 34.0 and 358.3, respectively. Maximums of activity were detected with when growing the producer on a medium containing only amine nitrogen sources (fish flour hydrolysate and peptone), however, the amount of extracellular protein and the specific fibrinolytic and total proteolytic activity were greater in the medium containing both mineral and amine nitrogen sources (fish flour hydrolysate and sodium nitrate), rather than on a medium containing fish flour hydrolysate and peptone as nitrogen sources.
About the Authors
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, 119234, Moscow, Russia
E. S. Zvonareva
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, 119234, Moscow, Russia
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, 119234, Moscow, Russia
N. A. Baranova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology
Leninskiye Gory 1–12, 119234, Moscow, Russia
N. S. Egorov
Lomonosov Moscow State University
Russian Federation
Russian Federation
International Biotechnology Center
Leninskiye Gory 1–12, 119234, Moscow, Russia
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Review
For citations:
Osmolovskiy A.A., Zvonareva E.S., Kreyer V.G., Baranova N.A., Egorov N.S. SECRETION OF PROTEINASES WITH FIBRINOLYTIC ACTIVITY BY MICROMYCETES OF THE GENUS ASPERGILLUS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(1):47-51. (In Russ.)
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