THE STUDY OF INTERACTION OF THE HP1 FAMILY PROTEINS WITH THE UNTRANSLATED REGULATORY REGIONS OF THE GYPSY RETROTRANSPOSONS OF DROSOPHILA MELANOGASTER
Abstract
We have investigated the in vitro interaction of recombinant HP1 family protein paralogs (HP1a, HP1b and HP1c) and the 5’ untranslated regulatory regions of gypsy retrotransposon group in Drosophila melanogaster: gypsy, Springer, Tirant, ZAM, Rover and 17.6. With the use of competitive DNA, conditions for specific binding were obtained. It is shown that HP1 family proteins effectively bind to the 5’ untranslated region of retrotransposons, which have tandem repeats in their composition. It has been found that repeats are absolutely necessary for binding HP1a protein to the 5’ untranslated region of Tirant and ZAM mobile genetic elements. The absence of repeats in the case of ZAM element or their number less than two—in the case of Tirant makes such interaction impossible. Thus, the presence of tandem repeats in the 5’ untranslated region of retrotransposons of the gypsy group is an important tool for regulating their transposition by heterochromatin proteins.
About the Authors
A. R. Lavrenov
Lomonosov Moscow State University
Russian Federation
Department of Genetics, Faculty of Biology
Russian Federation
Department of Genetics, Faculty of Biology
L. N. Nefedova
Lomonosov Moscow State University
Russian Federation
Department of Genetics, Faculty of Biology
Russian Federation
Department of Genetics, Faculty of Biology
A. I. Kim
Lomonosov Moscow State University
Russian Federation
Department of Genetics, Faculty of Biology
Russian Federation
Department of Genetics, Faculty of Biology
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Review
For citations:
Lavrenov A.R., Nefedova L.N., Kim A.I. THE STUDY OF INTERACTION OF THE HP1 FAMILY PROTEINS WITH THE UNTRANSLATED REGULATORY REGIONS OF THE GYPSY RETROTRANSPOSONS OF DROSOPHILA MELANOGASTER. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(2):63-71. (In Russ.)
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