FLUORESCENCE INDUCTION KINETICS IN THE MEMBRANE PREPARATION OF PHOTOSYSTEM II WITH HETEROGENЕOUS METAL CLUSTERS (Mn/Fe) IN THE OXYGEN-EVOLVING COMPLEX
Abstract
Transport of electrons in the spinach photosystem II (PSII) with oxygen-evolving complex (OEC) containing heterogenеous metal clusters 2Mn2Fe and 3Mn1Fe was investigated using the method of fluorescence induction kinеtic (FIK) measurement. Preparations of PSII(2Mn,2Fe) and PSII(3Mn,1Fe) were produced using Ca-depleted PSII membranes (PSII(-Ca)). We found that FIK in PSII(2Mn,2Fe) membranes are similar to FIK form in PSII(-Ca) samples but have lower fluorescence yield. Our results demonstrate the existence the electron transfer from metal cluster in the OEC to primary plastoquinone electron acceptor QА as well as in PSII(-Ca) preparations and show that substitution of Mn with Fe doesn’t effect on the electron transport on the PSII acceptor side. Thus these data demonstrate the possibility of water oxidation by heterogeneous metal cluster or water oxidation by only Mn dimer. We established that the form of FIK in PSII(3Mn,1Fe) preparations resembles the FIK in PSII(2Mn,2Fe) membranes. Yield of maximal fluorescence yield Fmax is larger but not significantly. The rate of electron transfer in the presence of Ca2+ rises significantly (2 times) whereas Ca2+ has no influence in PSII(2Mn,2Fe) membranes which don’t evolve oxygen. In the Mn-depleted PSII membranes FIK reach maximum (so-called peak K) then decreases as consequence of QА - oxidation and absence of electron input. Insertion of Fe cations instead of Mn provides the fluorescence saturation and disappearance of peak K possibly due to the delay of recombination process between reduced primary electron acceptor QА - and oxidized tyrosine YZ •+ – electron carrier between OEC and primary electron donor P680.
About the Authors
L. N. Davletshina
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, Faculty of Biology,
Leninskiye gory 1–12, Moscow 119234
B. K. Semin
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, Faculty of Biology,
Leninskiye gory 1–12, Moscow 119234
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Review
For citations:
Davletshina L.N., Semin B.K. FLUORESCENCE INDUCTION KINETICS IN THE MEMBRANE PREPARATION OF PHOTOSYSTEM II WITH HETEROGENЕOUS METAL CLUSTERS (Mn/Fe) IN THE OXYGEN-EVOLVING COMPLEX. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(3):141-145. (In Russ.)
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