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POSSIBILITIES OF OPTICAL MONITORING OF PHOSPHORUS STARVATION IN SUSPENSIONS OF THE MICROALGA CHLORELLA VULGARIS IPPAS C-1 (CHLOROPHYCEAE)

Abstract

Studies of the impact of inorganic phosphorus (Pi ), an important nutrient, on the growth and physiological parameters of single-celled algae are important for investigations of the dynamics of phytoplankton abundance and productivity in natural ecosystems as well as in industrial systems for the cultivation of microalgae. Difficulties in carrying out such studies are associated with the complex kinetics of Pi uptake by and the ability of microalgae to store phosphorus in their cells. This situation necessitates the efficient methods for express monitoring of microalgal cultures such as the methods based on the registration of optical properties of cells, such as absorption and scattering of light and fluorescence of chlorophyll contained in the cells. Here, we describe the results of monitoring the cultures of a chlorophyte Chlorella vulgaris IPPAS C-1, starving for phosphorus. It was found that both optical (light absorption in the bands of the key pigments–chlorophylls and carotenoids) and luminescent (variable fluorescence of chlorophyll) parameters reflect closely the culture condition. The correction for the contribution of light scattering to the overall extinction of light by microalgal cell suspensions turned to be necessary. At the same time, the light scattering signal is an accurate measure of the total number of suspended particles in the suspension. However, it is difficult to monitor via optical absorption the samples with abundant light-scattering particles lacking the photosynthetic pigments (such as heterotrophic bacteria). For such cultures, the using of variable fluorescence-based parameter Fv/Fm reflecting the maximum photochemical efficiency of the photosystem II is advisable.

About the Authors

A. G. Kuznetsov
Lomonosov Moscow State University
Russian Federation

Departments of Biophysics, Faculty of Biology,

119234, Moscow, Leninskiye Gory 1–12



S. I. Pogosyan
Lomonosov Moscow State University
Russian Federation

Departments of Biophysics, Faculty of Biology,

119234, Moscow, Leninskiye Gory 1–12



I. V. Konyukhov
Lomonosov Moscow State University
Russian Federation

Departments of Biophysics, Faculty of Biology, 

119234, Moscow, Leninskiye Gory 1–12



S. G. Vasilieva
Lomonosov Moscow State University
Russian Federation

Departments of Bioengineering, Faculty of Biology,

119234, Moscow, Leninskiye Gory 1–12;



A. A. Lukyanov
Lomonosov Moscow State University
Russian Federation

Departments of Bioengineering, Faculty of Biology,

119234, Moscow, Leninskiye Gory 1–12



V. S. Zotov
Federal Research Centre “Fundamentals of Biotechnology” RAS
Russian Federation
119071 Moscow, Leninskii Prospekt 33–2


L. Nedbal
Institute of Bio- and Geosciences 2: Plant Sciences (IBG-2), Forschungszentrum J lich (FZJ)
Russian Federation
52428 Jülich, Leo-Brandt str


A. E. Solovchenko
Lomonosov Moscow State University; Euarsian Center for Food Security
Russian Federation

Departments of Bioengineering, Faculty of Biology,

119234, Moscow, Leninskiye Gory, 1–12



References

1. Grossman A.R., Aksoy M. Algae in a phosphorus-limited landscape // Annual plant reviews. Vol. 48. Phosphorus metabolism in plants / Eds. W. Plaxton and H. Lambers. John Wiley & Sons, 2015. P. 337–374.

2. Solovchenko A., Verschoor A.M., Jablonowski N.D., Nedbal L. Phosphorus from wastewater to crops: An alternative path involving microalgae // Biotechnol. Adv. 2016. Vol. 34. N 5. P. 550–564.

3. Schreiber C., Schiedung H., Harrison L., Briese C., Ackermann B., Kant J., Schrey S.D., Hofmann D., Singh D., Ebenhöh O., Amelung W., Schurr U., Mettler-Altmann T., Huber G., Jablonowski N. D., Nedbal L. Evaluating potential of green alga Chlorella vulgaris to accumulate phosphorus and to fertilize nutrient-poor soil substrates for crop plants // J. Appl. Phycol. 2018. DOI 10.1007/s10811-018-1390-9.

4. Cembella A.D., Antia N.J., Harrison P.J. The utilization of inorganic and organic phosphorous compounds as nutrients by eukaryotic microalgae: A multidisciplinary perspective: Part I // Crit. Rev. Microbiol. 1982. Vol. 10. N 4. P. 317–391.

5. Antal T.K., Matorin D.N., Ilyash L.V., Volgusheva A.A., Osipov V.I., Konyuhov I.V., Krendeleva T.E., Rubin A.B. Probing of photosynthetic reactions in four phytoplanktonic algae with a PEA fluorometer // Photosynth. Res. 2009. Vol. 102. N. 1. P. 67–76.

6. Maxwell K., Johnson G. Chlorophyll fluorescence — a practical guide // J. Exp. Bot. 2000. Vol. 51. N 345. P. 659–668.

7. Matorin D.N., Antal T.K., Ostrowska M., Rubin A.B., Ficek D., Majchrowski R. Chlorophyll fluorimetry as a method for studying light absorption by photosynthetic pigments in marine algae // Oceanologia. 2004. Vol. 46. N 4. P. 519–531.

8. Solovchenko A., Gorelova O., Selyakh I., Pogosyan S., Baulina O., Semenova L., Chivkunova O., Voronova E., Konyukhov I., Scherbakov P. A novel CO2-tolerant symbiotic Desmodesmus (Chlorophyceae, Desmodesmaceae): Acclimation to and performance at a high carbon dioxide level // Algal Res. 2015. Vol. 11. P. 399–410.

9. Solovchenko A., Pogosyan S., Chivkunova O., Selyakh I., Semenova L., Voronova E., Scherbakov P., Konyukhov I., Chekanov K., Kirpichnikov M., Lobakova E. Phycoremediation of alcohol distillery wastewater with a novel Chlorella sorokiniana strain cultivated in a photobioreactor monitored on-line via chlorophyll fluorescence // Algal Res. 2014. Vol. 6. Part B. P. 234–241.

10. Solovchenko A., Merzlyak M., Khozin-Goldberg I., Cohen Z., Boussiba S. Coordinated carotenoid and lipid syntheses induced in Parietochloris incisa (Chlorophyta, Trebouxiophyceae) mutant deficient in Δ5 desaturase by nitrogen starvation and high light // J. Phycol. 2010. Vol. 46. N 4. P. 763–772.

11. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria // J. Gen. Microbiol. 1979. Vol. 111. N 1. P. 1–61.

12. Nagul E.A., McKelvie I.D., Worsfold P., Kolev S.D. The molybdenum blue reaction for the determination of orthophosphate revisited: opening the black box // Analyt. Chim. Acta. 2015. Vol. 890. P. 60–82.

13. Merzlyak M.N., Naqvi K.R. On recording the true absorption spectrum and the scattering spectrum of a turbid sample: application to cell suspensions of the cyanobacterium Anabaena variabilis // J. Photochem. Photobiol. B. Biol. 2000. Vol. 58. N 2–3. P. 123–129.

14. Solovchenko A., Khozin-Goldberg I., Cohen Z., Merzlyak M. Carotenoid-to-chlorophyll ratio as a proxy for assay of total fatty acids and arachidonic acid content in the green microalga Parietochloris incisa // J. Appl. Phycol. 2009. Vol. 21. N 3. P. 361–366.

15. Merzlyak M.N., Chivkunova O.B., Maslova I.P., Naqvi K.R., Solovchenko A.E., Klyachko-Gurvich G.L. Light absorption and scattering by cell suspensions of some cyanobacteria and microalgae // Russ. J. Plant Physiol. 2008. Vol. 55. N 3. P. 464–470.

16. Aitchison P., Butt V. The relation between the synthesis of inorganic polyphosphate and phosphate uptake by Chlorella vulgaris // J. Exp. Bot. 1973. Vol. 24. N 3. P. 497–510.

17. Ruban A. V. Non-photochemical chlorophyll fluorescence quenching: mechanism and effectiveness in protection against photodamage // Plant Physiol. 2016. Vol. 170. N 4. P. 1903–1916.

18. Horton P. Developments in research on non-photochemical fluorescence quenching: Emergence of key ideas, theories and experimental approaches // Non-photochemical quenching and energy dissipation in plants, algae and cyanobacteria / Eds. B. Demmig-Adams, G. Garab,W. Adams III, and Govindjee. Dordrecht: Springer Netherlands, 2014. P. 73–95.

19. Shibata K. Dual wavelength scanning of leaves and tissues with opal glass // Biochim. Biophys. Acta. 1973. Vol. 304. N 2. P. 249–259.


Review

For citations:


Kuznetsov A.G., Pogosyan S.I., Konyukhov I.V., Vasilieva S.G., Lukyanov A.A., Zotov V.S., Nedbal L., Solovchenko A.E. POSSIBILITIES OF OPTICAL MONITORING OF PHOSPHORUS STARVATION IN SUSPENSIONS OF THE MICROALGA CHLORELLA VULGARIS IPPAS C-1 (CHLOROPHYCEAE). Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(3):146-152. (In Russ.)

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