CELL KINETIC APPROACHES TO THE SEARCH FOR ANTI-AGING DRUGS: THIRTY YEARS AFTER
Abstract
A brief overview of the ideas of the possibility of using the cell kinetic model developed by the author in the 1980s to test, in experiments on cell cultures, potential geroprotectors and geropromoters, which slow down or accelerate, respectively, the aging of animals and humans. The process of the evolution of this model is considered – from the estimation of only the cell reproduction rate and saturation density in a non-subcultured cell culture to the constructing of survival curves in the stationary phase of growth, and further – to an analysis of the possible interrelation between all parts of the curve of cells’ growth and subsequent dying out. Possible approaches to mathematical and statistical analysis of the data obtained within the framework of this model system are analyzed. It is emphasized that such studies can be carried out on cells of very different nature (normal and transformed human and animal cells, plant cells, yeast, mycoplasmas, bacteria, etc.), which makes possible an evolutionary approach to the interpretation of the results obtained. At the same time, in the author’s opinion, the most promising experiments are those carried out on immortalized cells of humans and animals, since they are not cancerous on the one hand, and on the other have an unlimited mitotic potential and, therefore, do not “age” in the process of numerous divisions, as, for example, normal human diploid fibroblasts do. It is assumed that the appropriate mathematical analysis of the entire growth and dying out curve of a non-subcultured cell culture (from seeding into a culture flask to the complete death of all cells) may allow us to clarify certain relationships between the development and aging of a multicellular organism, and to increase the reliability of identifying promising geroprotectors.
About the Author
A.N. Khokhlov A.N. Khokhlov A.N. Khokhlov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Leninskiye gory 1–12, Moscow, 119234
Evolutionary Cytogerontology Sector, School of Biology
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A.N. Khokhlov A.A. CELL KINETIC APPROACHES TO THE SEARCH FOR ANTI-AGING DRUGS: THIRTY YEARS AFTER. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2018;73(4):227-232. (In Russ.)
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