PCR IDENTIFICATION OF PATHOGENIC AGROBACTERIES DETECTED ON VINEYARDS OF KRASNODAR TERRITORY BY THE TYPE OF CONTAINED Ti PLASMIDS
Abstract
Agrobacterium vitis and some strains of A. tumefaciens and A. rhizogenes cause a dangerous chronic disease on grape plants – crown gall. Using molecular genetic methods, work has been carried out to identify and study the diversity of pathogenic agrobacteria that cause crown gall in the vineyards of the Krasnodar Territory. 76 samples of young and lignified tumor-like outgrowths of crown gall of grapes collected from plants in fourteen vineyards of the Krasnodar Territory and having a different origin of planting material (Italy, France, Austria, Serbia, Bulgaria, Russia) were used as material. In all studied samples, the presence of A. vitis was identified using the PGF/PGR test-system for DNA analysis. When studying the diversity of agrobacteria by the type of Ti plasmids contained therein, four groups were identified: containing octopine-type Ti plasmids (59.5%), octopine/vitopine (13.5%), vitopine (13.5%) and nopaline type (13.5%). The obtained data indicate that in the vineyards of the Krasnodar Territory crown gall of grapes is caused by agrobacteria A. vitis, mainly of the octopine type.
About the Authors
M. V. Makarkina
North-Caucasian federal scientific center of horticulture, viticulture, wine-making
Russian Federation
Russian Federation
40 years of Victory street, 39, Krasnodar, 350901
E. T. Ilnitskaya
North-Caucasian federal scientific center of horticulture, viticulture, wine-making
Russian Federation
Russian Federation
40 years of Victory street, 39, Krasnodar, 350901
S. V. Tokmakov
North-Caucasian federal scientific center of horticulture, viticulture, wine-making
Russian Federation
Russian Federation
40 years of Victory street, 39, Krasnodar, 350901
References
1. Gelvin S.B. Agrobacterium-mediated plant transformation: The biology behind the “genejockeying” tool // Microbiol. Mol. Biol. Rev. 2003. Vol. 67. N 1. P. 16–37.
2. Tarbah F.A., Goodman R.N. Rapid detection of Agrobacterium tumefaciens in grapevine propagating material and the basis for an efficient indexing system // Plant Dis. 1986. Vol. 70. P. 566–568
3. Бурдинская В.Ф., Арестова Н.О. Латентная зараженность винограда бактериальным раком // Защ. карант. раст. 2010. № 10. С. 38– 39
4. Kerr A., Panagopoulos C.G. Biotypes of Agrobacterium radiobacter var. tumefaciens and their biological control // J. Phytopathol. 1977. Vol. 90. N 2. P. 172–179.
5. Ophel K., Kerr A. Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines // Int. J. Syst. Bacteriol. 1990. Vol. 40. N 3. P. 236–241.
6. Mousavi S.A, Osterman J, Wahlberg N., Nesme X., Lavire C., Vial L., Paulin L., de Lajudie P., Lindstrom K. Phylogeny of the Rhizobium-Allorhizobium-Agrobacterium clade supports the delineation of Neorhizobium gen. nov // Syst. Appl. Microbiol. 2014. Vol. 37. N 3. P. 208–215.
7. Ramírez-Bahena M.H., Vial L., Lassalle F., Diel B., Chapulliot D., Daubin V., Nesme X., Muller D. Single acquisition of protelomerase gave rise to speciation of a large and diverse clade within the Agrobacterium/Rhizobium supercluster characterized by the presence of a linear chromid // Mol. Phylogenet. Evol. 2014. Vol. 73. P. 202– 207.
8. Slater S.C., Goldman B.S., Goodner B. et al. Genome sequences of three agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria // J. Bacteriol. 2009. Vol. 191. N 8. P. 2501–2511.
9. Szegedi E. Opines in naturally infected crown gall tumors // Vitis. 2003. Vol. 42. N 1. P. 39–41.
10. Haas J.H, Moore L.W., Ream W., Manulis S. Universal PCR primers for detection of phytopathogenic Agrobacterium strains // Appl. Environ. Microbiol. 1995. Vol. 61. N 8. P. 2879– 2884.
11. Матвеева Т.В., Богомаз Д.И., Лутова Л.А. Способ диагностики биоматериалов на наличие в них агробактерий. Патент РФ на изобретение № 2458142. Дата публ.: 10.08.12. Бюл. №22/12.
12. Puławska J., Willems A., Sobiczewski P. Rapid and specific identification of four Agrobacterium species and biovars using multiplex PCR // Syst. Appl. Microbiol. 2006. Vol. 29. N. 6. P. 470–479.
13. Sawada H., Ieki H., Matsuda I. PCR detection of Ti and Ri plasmid from phytopathogenic Agrobacterium strains // Appl. Environ. Microbiol. 1995. Vol. 61. N 2. P. 828– 831.
14. Suzaki K., Yoshida K., Sawada H. Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers // J. Gen. Plant. Pathol. 2004. Vol. 70. N 6. P. 342–347.
15. Bini F, Kuczmog A., Putnoky P. Novel pathogen-specific primers for the detection of Agrobacterium vitis and Agrobacterium tumefaciens // Vitis. 2008. Vol. 47. N 3. P. 181–190.
16. Eastwell K.C., Kenneth C., Leslie Willis G., Cavileer D.T. A rapid and sensitive method to detect Agrobacterium vitis in grapevine cuttings using the polymerase chain reaction // Plant Dis. 1995. Vol. 79. N 8. P. 822–827.
17. Herlache T.C., Hotchkiss A.T., Burr T.J., Collmer A. Characterization of the Agrobacterium vitis pehA gene and comparison of the encoded polygalacturonase with the homologous enzymes from Erwinia carotovora and Ralstonia solanacearum // Appl. Environ. Microbiol. 1997. Vol. 63. N 1. P. 338–346.
18. Kawaguchi A., Sawada,H., Inoue, K., Nasu H. Multiplex PCR for the identification of Agrobacterium biovar 3 strains // J. Gen. Plant Pathol. 2005. Vol. 71. N 1. P. 54–59.
19. Lim S.H., Kim J.G., Kang H.W. Novel SCAR primers for specific and sensitive detection of Agrobacterium vitis strains // Microbiol. Res. 2009. Vol. 164. N. 4. P. 451–460.
20. Canaday J., Gerard J.C., Crouzet P., Otten L. Organization and functional analysis of three T-DNAs from the vitopine Ti plasmid pTiS4 // Mol. Gen. Genet. 1992. Vol. 235. N 2–3. P. 292–303.
21. Szegedi E., Bottka S. Detection of Agrobacterium vitis by polymerase chain reaction in grapevine bleeding sap after isolation on a semiselective medium // Vitis. 2002. Vol. 41. N 1. P. 37–42.
22. Szegedi E., Bottka S., Mikulás J., Otten L., Sule S. Characterization of Agrobacterium tumefaciens strains isolated from grapevine // Vitis. 2005. Vol. 44. N 1. P. 49–54.
23. Bini F., Geider K., Bazzi C. Detection of Agrobacterium vitis by PCR using novel virD2 gene-specific primers that discriminate two subgroups // Eur. J. Plant Pathol. 2008. Vol. 122. N 3. P. 403–411.
24. Argun N., Momol M.T., Maden S., Momol E.A., Reid C.L., Celek H., Burr T.J. Characterization of Agrobacterium vitis strains isolated from Turkish grape cultivars in the Central Anatolia region // Plant Dis. 2002. Vol. 86. N 2. P. 162–166.
25. Lamovšek J., Zidarič I., Pleško I.M., Urek G., Trdan S. Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia // Acta Agric. Slov. 2015. Vol. 103. N 2. P. 313–321.
26. Genov I., Atanassov I., Tsvetkov I., Atanassov A. Isolation and characterization of Agrobacterium strains from grapevines in Bulgarian vineyards and wild grapes, V. vinifera ssp. Silvestris // Vitis. 2015. Vol. 45. N 2. P. 97– 101.
27. Winans S.C., Allenza P., Stachel S.E., McBride K.E., Nester E.W. Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6 // Nucleic Acids Res. 1987. Vol. 15. N 2. P. 825–837.
28. Tolba I.H., Zaki M.F. Characterization of Agrobacterium vitis isolates obtained from galled grapevine plants in Egypt // Ann. Agric. Sci. 2011. Vol. 56. N 2. P. 113–119.
29. Kuzmanović N., Gašić K., Ivanović M., Prokić A., Obradović A. Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia. Arch. Biol. Sci. 2012. Vol. 64. N 4. P. 1487–1494.
30. Rouhrazi K., Rahimian H. Characterization of Iranian grapevine isolates of Rhizobium (Agrobacterium) spp. // J. Plant Pathol. 2012. Vol. 94. N 3. P. 555–560.
31. Filo A., Sabbatini P., Sundin G.W., Zabadal T.J., Safe G.R., Cousins P.S. Grapevine crown gall suppression using biological control and genetic engineering: a review of recent research // Am. J. Enol. Vitic. 2013. Vol. 64. N. 1. DOI 10.5344/ajev.2012.12038.
32. Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh tissue // Phytochem. Bull. 1987. Vol. 19. P. 11– 15.
33. Makarkina M.V., Ilnitskaya E.T., Stepanov I.V. Identification of Agrobacteria on grape plants with symptoms of crown gall lesions in ampeloсenosises of Krasnodar Territory using the PCR method // Russ. Agric. Sci. 2017. Vol. 43. N 5. P. 402–405.
34. Макаркина М.В., Ильницкая Е.Т. Определение латентной стадии бактериального рака винограда методом полимеразной цепной реакции // Научные труды Северо-Кавказского федерального научного центра садоводства, виноградарства, виноделия. Т. 13. Краснодар, 2017. С. 100–104.
35. Ignatov A.N., Khodykina M.V., Vinogradova S.V., Polityko V.A., Kornev K.P., Mazurin E.S. First report of Agrobacterium vitis causing crown galls of wine grape in Russia // Plant Dis. 2016. Vol. 100. N. 4. P. 853.
Review
For citations:
Makarkina M.V., Ilnitskaya E.T., Tokmakov S.V. PCR IDENTIFICATION OF PATHOGENIC AGROBACTERIES DETECTED ON VINEYARDS OF KRASNODAR TERRITORY BY THE TYPE OF CONTAINED Ti PLASMIDS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2019;74(1):50-58. (In Russ.)
Views:
465
Email this article 