PARP1 binding to DNA breaks and hairpins alters nucleosome structure
Abstract
DP-ribose)polymerase 1 (PARP1) is involved in the processes of DNA repair, replication, transcription, cell cycle regulation and apoptosis. Participation of PARP 1 in DNA repair is determined by the ability of the enzyme to interact with various defects and non-canonical structures of DNA with consequent polyADP- ribosylation of neighboring proteins. Earlier for mononucleosomes containing a DNA end recapitulating double-strand DNA break near the nucleosome, we found that PARP1 induces nucleosome structural changes in the absence of NAD+. In the present work we report that PARP1 induces similar structural changes in nucleosomes containing either DNA ends extending from the core by 20 b.p. or containing hairpins at the DNA ends. In all the cases PARP1 caused changes in DNA wrapping on the surface of the histone octamer that are accompanied by an increase in the distance between adjacent DNA gyres. These PARP1-mediated changes in the nucleosome structure presumably contribute to chromatin decondensation and facilitate access of repair enzymes to damaged DNA.
About the Authors
N. V. Malyuchenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Bioengineering Department, Biological Faculty,
Leninskie Gory 1–12, Moscow, 119234
E. Yu. Kotova
Cancer Epigenetics Team, Fox Chase Cancer Center
United States
Cottman Avenue 333, Philadelphia, PA 19111
United States
Cottman Avenue 333, Philadelphia, PA 19111
M. P. Kirpichnikov
Lomonosov Moscow State University; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation
Leninskie Gory 1–12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, 117997, Moscow
Russian Federation
Leninskie Gory 1–12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, 117997, Moscow
V. M. Studitsky
Lomonosov Moscow State University; Cancer Epigenetics Team, Fox Chase Cancer Center
United States
Leninskie Gory 1–12, Moscow, 119234; Cottman Avenue 333, Philadelphia, PA 19111
United States
Leninskie Gory 1–12, Moscow, 119234; Cottman Avenue 333, Philadelphia, PA 19111
A. V. Feofanov
Lomonosov Moscow State University; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation
Leninskie Gory 1–12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, 117997, Moscow
Russian Federation
Leninskie Gory 1–12, Moscow, 119234; ul. Miklukho-Maklaya 16/10, 117997, Moscow
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Review
For citations:
Malyuchenko N.V., Kotova E.Yu., Kirpichnikov M.P., Studitsky V.M., Feofanov A.V. PARP1 binding to DNA breaks and hairpins alters nucleosome structure. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2019;74(3):200-206. (In Russ.)
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