Study of effect of «mild uncoupling» by 2,4-dinitrophenol on growth and death of culture of Chinese hamster cells in the stationary phase

Partial uncoupling of processes of oxidative phosphorylation and energy storage in the form of ATP («mild» uncoupling) helps reduce the production of reactive oxygen species and can also mimic the effect of calorie restriction. A number of studies have shown that uncouplers like 2,4-dinitrophenol (DNF) affect the lifespan of Drosophila, yeast, mice and rats, as well as the manifestation of «age-related» changes in replicative senescence mammalian and human cell cultures. This paper is devoted to studying the effect of DNF on the growth and death of «stationary phase aging» Chinese hamster cells. Using the method for determining the colony-forming efficiency of cells, the maximum permissible concentration was selected, 5•10-5 M, in which the substance potentially contributes to the manifestation of the effect of «mild» uncoupling and does not inhibit cell proliferation. At higher concentrations, DNP has a cytotoxic effect for the studied cell culture. Under the influence of DNP, the kinetics of cell growth and cell death does not change in the potentially «mild» uncoupling concentration (5.6•10-7 M), the lifespan of the cell culture does not increase. A similar effect may be due to the type of cells studied. In addition, there is a probability that the opt  l concentration lies in the range from 5•10-7 to 5•10-5 M, or lower than 5•10-7 M.

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