Analysis of element composition of DNA-protein crystals in vitro
Abstract
The universal response of Escherichia coli to stress is the enhancement of the synthesis of specific histone-like Dps proteins that bind bacterial DNA. As a result, two-dimensional and three-dimensional crystalline arrays can be observed in the cytoplasm of starving bacteria. We selected the conditions for obtaining in vitro cocrystals of DNA-Dps and studied their elemental composition using analytical electron microscopy. We found that Dps in the co-crystal retains its ferritin-like activity, that is, it can stimulate the oxidation of Fe2+ ions to Fe3+ and facilitate the accumulation of iron in the form of Fe2O3 in the inner cavity of the oligomer.
About the Authors
A. V. Moiseenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology Lomonosov MSU.
Leninskiye Gory 1—12, Moscow, 119234
N. G. Loiko
Research Center of Biotechnology, Russian Academy of Sciences; N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences
Russian Federation
Russian Federation
Leninsky prospect 33—2, Moscow, 119071; Kosygina st., 4, Moscow, 117977
O. V. Chertkov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology Lomonosov MSU
Leninskiye Gory 1—12, Moscow, 119234
A. V. Feofanov
Lomonosov Moscow State University; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology Lomonosov MSU
Leninskiye Gory 1—12, Moscow, 119234; Miklouho-Maclay st. 16/10 Moscow, 117997
Yu. F. Krupyanskii
N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences
Russian Federation
Russian Federation
Kosygina st., 4, Moscow, 117977
O. S. Sokolova
Lomonosov Moscow State University; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology Lomonosov MSU.
Leninskiye Gory 1—12, Moscow, 119234; Miklouho-Maclay st. 16/10 Moscow, 117997
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Review
For citations:
Moiseenko A.V., Loiko N.G., Chertkov O.V., Feofanov A.V., Krupyanskii Yu.F., Sokolova O.S. Analysis of element composition of DNA-protein crystals in vitro. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2019;74(4):301–307. (In Russ.)
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