The interaction peculiarities in vitro OF osteoblast-like cells mg-63 with surface of Ti-Zr-Nb shape memory alloy

The paper studies the influence of the Ti-Zr-Nb (TZN) shape memory alloys surface on adhesion, proliferation, viability and actin cytoskeleton organization of osteoblast-like cells MG-63. The studied materials have a unique combination of mechanical properties that determine their prospects for creating bone implants with high biomechanical compatibility: low value of the Young’s modulus and superelastic behavior, similar to the behavior of bone tissue. We used thin plates of the experimental alloy TZN and the Ti-Al-Nb medical alloy (TAN) as a control material. A study of the growth dynamics of the MG-63 cell culture was made using the MTT test and counting the number of nuclei per unit area using scanning microscopy. It was found that on 4 and 7 days the number of cells on the TZN alloy is higher than on the TAN alloy. This may be due to the influence of the qualitative and quantitative composition of materials on the surface microstructure and chemistry. The viability over the cultivation time was close to 100% on both alloys. The analysis of cytoskeleton images showed the predominance of fibrillary actin on samples of the TZN system, as well as the organization characteristic of fibroblast-like polygonal cells.

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