NEW MODEL SYSTEM FOR THE STUDY OF ANIMAL IMMUNITY TO FUNGAL INFECTIONS
https://doi.org/10.1234/XXXX-XXXX-2014-2-3-9
Abstract
The association of the model organism Caenorhabditis elegans and fungus Pleurotus ostreatus gives the possibility to study the molecular and genetic mechanisms of the early stages of the spatial and temporal interactions of animals with fungal pathogens. We identified the initial contacts of P. ostreatus and the nematode C. еlegans. We found that the fungus paralyzed C. еlegans, penetrated into their alive bodies either through natural openings or directly by piercing the cuticle and colonized them. We offer the new model host-pathogen association to study further the local defense reactions of C. elegans against fungal infection.
About the Authors
J. M. Plotnikova
Отдел молекулярной биологии ГМ и Отдел генетики Гарвардского университета, Бостон, США
Russian Federation
Russian Federation
O. V. Kamzolkina
Russian Federation
F. M. Ausubel
Отдел молекулярной биологии ГМ и Отдел генетики Гарвардского университета, Бостон, США
References
1. Brenner S. The genetics of Caenorhabditis elegans // Genetics. 1974. Vol. 77. N 1. P. 71—94.
2. Hall D., Altun Z.F. C. elegans. Atlas. N.Y.: Cold Spring Harbor, 2007. 348 p.
3. McGhee J.D. The C. elegans intestine. N.Y: WormBook, 2007. P. 1—36.
4. Kosinski R.A., Zaremba M. Dynamics of the model of the Caenorhabditis elegans Neural Network // Acta Physica Polonica. 2007. Vol. B38. N 6. P. 2202.
5. Powell J.R., Ausubel F.M. Models of Caenorhabditis elegans infection by bacterial and fungal pathogens // Methods Mol. Biol. 2008. Vol. 415. P. 403—427.
6. The C. elegans sequencing consortium, consortium. Genome sequence of the nematode C. elegans: a platform for investigating biology // Science. 1998. Vol. 282. N 5396. P. 2012—2018.
7. Sifri C.D., Begun J., Ausubel F.M. The worm has turned — microbial virulence modeled in Caenorhabditis elegans // Trends in Microbiology. 2005. Vol. 13. P. 119—127.
8. Pujol N., Link E.M., Liu L.X., Kurz C.L., Alloing G., Tan M.W., Ray K.P., Solari R., Johnson C.D., Ewbank J.J. A reverse genetic analysis of components of the Toll signaling pathway in Caenorhabditis elegans // Curr. Biol. 2001. Vol. 11. P. 809—821.
9. Irazoqui J.E., Troemel E.R., Feinbaum R.L. et al. Distinct pathogenesis and host responses during infection of C. elegans by P. aeruginosa and S. aureus // PLoS Pathogens. 2008. Vol. 6. P. 7.
10. Irazoqui J.E., Ausubel F.M. Caenorhabditis elegans as a model to study tissues involved in host immunity and microbial pathogenesis // 99th Dahlem Conference on Infection, Inflammation and Clinic Inflammatoty Disorders: Clinical and Exp. Immunology. 2010. Vol. 160. P. 48—57.
11. Hans-Borje J. Adhesion of conidia of Drechmeria coniospora to Caenorhabditis elegans Wild Type and Mutants // J. Nematol. 1994. Vol. 26. N 4. P. 430—435.
12. Pujol N., Cypowyj S., Ziegler K., Millet A., Astrain A., Goncharov A., Jin Y., Chisholm A., Ewbank J.J. Distinct innate immune responses to infection and wounding in the C. elegans epidermis // Curr Biol. 2008. Vol. 18. P. 481—489.
13. Thorn R.G., Barron G.L. Carnivorous fungi // Science. 1984. Vol. 224. N 4644. P. 76—78.
14. Barron G.L. Predatory fungi, wood decay, and the carbon cycle // Biodiversity. 2003. Vol. 4. P. 3—9.
15. Гарибова Л.В., Сидорова И.И. Грибы. Энциклопедия природы России. М., 1999. 352 с.
16. Новосёлова Д.Н., Камзолкина О.В. Культивирование Pleurotus ostreatus (Jacq.) P. Kumm. совместно с дрожжами // Вестн. Моск. ун-та. Сер. 16. Биология. 2011. № 3. С. 25—28.
17. Kwok O.C.H., Plattner R., Weisleder D., Wicklow D.T. A Nematicidal toxin from Pleurotus ostreatus NRRL 35261 // J. Chem. Ecol. 1992. Vol. 18. N 2. P. 127—136.
18. http://genome.jgi-psf.org/PleosPC15_1/PleosPC15_1. home.html
19. http://www.indexfungorum.org
20. Курсанов Л.И. Микология. М.: Сельхозгиз, 1933. 36 c.
Review
For citations:
Plotnikova J.M., Kamzolkina O.V., Ausubel F.M. NEW MODEL SYSTEM FOR THE STUDY OF ANIMAL IMMUNITY TO FUNGAL INFECTIONS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2014;(2):3-9. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2014-2-3-9
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