Secretion of proteinase with activity that is similar to activity of proteins of the hemostatic system by micromycetes
Abstract
Using the chromogenic peptide substrates, proteolytic activity against proteins of the hemostasis system was studied in 9 micromycetes belonging to different ecological-trophic groups: phyto-, nemato-, myco- and entomopathogens, as well as human pathogens. It is shown that the investigated micromycetes secrete proteases with broad substrate specificity, but among them there are producers with pronounced both plasmin-like and urokinase (Arthrobotrys longa 1, Sarocladium strictum 203) also as TAP-like (Purpureocillium lilacinum k1) activity. The most active producer of proteases with fibrinolytic and activator activity to plasminogen activity was S. strictum 203 micromycete. The activity of proteinases formed by S. strictum 203 is about 50% of the fibrinolytic activity of plasminogen, which makes it a promising producer of fibrinolytic enzymes.
About the Authors
A. A. Lukianova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology,
Leninskiye gory 1–12, Moscow, 119234
E. I. Kornienko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology,
Leninskiye gory 1–12, Moscow, 119234
P. A. Vigand
Paris-Sacley University
France
Route de l’Orme aux Merisiers, RD 128, 91190, Saint-Aubin
France
Route de l’Orme aux Merisiers, RD 128, 91190, Saint-Aubin
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology,
Leninskiye gory 1–12, Moscow, 119234
A. V. Kurakov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Mycology and Algology, Faculty of Biology,
Leninskiye gory 1–12, Moscow, 119234
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology,
Leninskiye gory 1–12, Moscow, 119234
References
1. Kotb E. The biotechnological potential of fibrinolytic enzymes in the dissolution of endogenous blood thrombi // Biotechnol. Prog. 2014. Vol. 30. N 3. P. 656–672.
2. Kotb E., Helal G.E.-D.A., Edries F.M. Screening for fibrinolytic filamentous fungi and enzymatic properties of the most potent producer, Aspergillus brasiliensis AUMC 9735 // Biologia. 2015. Vol. 70. N 12. P. 1565–1574.
3. Sharkova T.S., Kurakov A.V., Osmolovskiy A.A., Matveeva E.O., Kreyer V.G., Baranova N.A., Egorov N.S. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes // Microbiology. 2015. Vol. 84. N 3. P. 359–364.
4. Osmolovskiy A.A., Kurakov A.V., Kreyer V.G., Baranova N.A., Egorov N.S. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus and Aspergillus sydowii to affect proteins of the human haemostatic system // Moscow Univ. Biol. Sci. Bull. 2017. Vol. 72. N 1. P. 20–24.
5. Шаркова Т.С., Матвеева Э.О., Кураков А.В., Осмоловский А.А., Крейер В.Г., Баранова Н.А., Егоров Н.С. Энтомопатогенные микромицеты – перспективные продуценты протеиназ с фибринолитической активностью // Усп. мед. микол. 2015. Т. 14. С. 458–460.
6. Kim H.C., Choi B.-S., Sapkota K., Kim S., Lee H.J., Yoo J.C., Kim S.-J. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Paecilomyces tenuipes // Process Biochem. 2011. Vol. 46. N 8. P. 1545–1553.
7. Osmolovskiy A.A., Kreyer V.G., Baranova N.A., Egorov N.S. Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete // Applied Biochem. Microbiol. 2017. Vol. 53. N 4. P. 429–434.
8. Batomunkueva B.P., Egorov N.S. Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities // Microbiology. 2001. Vol. 70. N 5. P. 519–522.
9. Osmolovskiy A.A., Rukavitsyna E.D., Kreier V.G., Baranova N.A., Egorov N.S. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus // Microbiology. 2017. Vol. 86. N 4. P. 512–516.
10. Proteolytic Enzymes. A practical approach / Ed. R. Beynon and J.S. Bond. Oxford: Oxford Univ. Press, 2001. 340 pp.
11. Егоров Н.С., Ландау Н.С., Буяк Л.И., Крейер В.Г. Гидролитическая система нокардиоформной бактерии Nocardia minima в процессе ее роста, развития и дифференциации // Микробиология. 1991. Т. 60. N 4. С. 637–643.
12. Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1 // Moscow Univ. Biol. Sci. Bull. 2016. Vol. 71. N 1. P. 62–66.
13. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding // Anal. Biochem. 1976. Vol. 72. N 1–2. P. 248–254.
14. Blieva R.K., Safuani Zh.E., Iskakbaeva Zh.A. Effect of various sources of nitrogen and carbon on the biosynthesis of proteolytic enzymes in a culture of Aspergillus awamori 21/96 // Applied Biochem. Microbiol. 2003. Vol. 39. N 2. P. 188–191.
15. Sharkova T.S., Kornienko E.I., Osmolovskii A.A., Kreier V.G., Baranova N.A., Egorov N.S. Morphological and physiological properties of the micromycete Arthrobotrys longa, a producer of Longolytin, a proteolytic complex with a thrombolytic effect // Microbiology. 2016. Vol. 85. N 2. P. 180–184.
16. Dias B.A., Neves P.M.O.J., Furlaneto-Maia L., Furlaneto M.C. Cuticle-degrading proteases produced by the entomopathogenic fungus Beauveria bassiana in the presence of coffee berry borer cuticle // Braz. J. Microbiol. 2008. Vol. 39. N 2. P. 301–306.
17. Umetsu H., Hishinuma K., Wake H., Ichishima E. Production, purification, and properties of serine carboxypeptidase from Paecilomyces carneus // Curr. Microbiol. 1996. Vol. 33. N 1. P. 44–48.
18. Scorsetti A.C., Elíades L.A., Stenglein S.A., Cabello M.N., Pelizza S.A., Saparrat M.C. Pathogenic and enzyme activities of the entomopathogenic fungus Tolypocladium cylindrosporum (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina // Rev. Biol. Trop. 2012. Vol. 60. N 2. P. 833–841.
19. Elad Y., Kapat A. The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea // Europ. J. Plant Pathol. 1999. Vol. 105. N 2. P. 177–183.
20. Kotlova E.K., Ivanova N.M., Yusupova M.P., Voyushina T.L., Ivanushkina N.E., Chestukhina G.G. Thiol-dependent serine proteinase from Paecilomyces lilacinus: Purification and catalytic properties // Biochemistry (Mosc.). 2007. Vol. 72. N 1. P. 117–123.
21. El-Aassar S.A., El-Badry H.M., Abdel-Fattah A.F. The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9 // Appl. Microbiol. Biotechnol. 1990. Vol. 33. N 1. P. 26–30.
22. Osmolovskiy A.A., Zvonareva E.S., Kreyer V.G., Baranova N.A., Egorov N.S. Secretion of proteinases with fibrinolytic activity by micromycetes of the genus Aspergillus // Moscow Univ. Biol. Sci. Bull. 2018. Vol. 73. N 1. P. 39–42.
Review
For citations:
Lukianova A.A., Kornienko E.I., Vigand P.A., Kreyer V.G., Kurakov A.V., Osmolovskiy A.A. Secretion of proteinase with activity that is similar to activity of proteins of the hemostatic system by micromycetes. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2020;75(1):37-42. (In Russ.)
Views:
345
Email this article 