The use of ISSR markers for the analysis of polymorphism in sea buckthorn (Hippophaë rhamnoides l.) Of different origin
Abstract
Sea buckthorn (Hippophaë rhamnoides L.) is a dioecious shrub, or tree. It is the perennial plant, the juvenile period of which continues from 3 to 5 years. Sea buckthorn is widely used in cosmetology, biotechnology, pharmacology, agriculture. It grows in many countries of Europe and Asia. This crop is a source of different vitamins, minerals, carotenoids and flavonoids. Phenotypic description does not give an accurate description of the taxon (species, subspecies, varieties, populations) so it means that we have to use molecular methods of analysis. The collection of Sea buckthorn with different ecological and geographical genesis was selected. It consists of herbarium specimens and natural populations. We selected ISSR-markers which are able to detect genotypic differences effectively. The dendrogram was built on the PCR basis. We revealed a high level of interspecies polymorphism, and it was also established that samples with similar ecological-geographical genesis tend to group into clusters, which are located at different genetic distances. The result of the research will help us to evaluate the gene pool of this crop, the diversity of forms, the similarity between them and it will help us to determine ecological and geographical origin of sea buckthorn, its evolution. So we will be able to select pairs for crossing and to define certification and identification of varieties before they reach the generative period.
About the Authors
K. D. Bone
All-Russia Research Institute of Agricultural Biotechnology;
Russian State Agrarian University—Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Laboratory of Plant Genetic Engineering, Timiryazevskaya st., 42, Moscow, 1275550;
Faculty of Agronomy and Biotechnology, Department of Genetics, Biotechnology, Breeding and Seed Production, Timiryazevskaya st., 49, Moscow, 1275550
I. V. Bocharkina
All-Russia Research Institute of Agricultural Biotechnology
Russian Federation
Russian Federation
Laboratory of Applied Genomics and Crop Breeding,
Timiryzevskay str. 42, Moscow, 127550
G. I. Karlov
All-Russia Research Institute of Agricultural Biotechnology
Russian Federation
Russian Federation
Laboratory of Plant Genetic Engineering,
Timiryazevskaya st., 42, Moscow, 1275550
O. V. Razumova
All-Russia Research Institute of Agricultural Biotechnology
Russian Federation
Russian Federation
Laboratory of Applied Genomics and Crop Breeding,
Timiryzevskay str. 42, Moscow, 127550
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Review
For citations:
Bone K.D., Bocharkina I.V., Karlov G.I., Razumova O.V. The use of ISSR markers for the analysis of polymorphism in sea buckthorn (Hippophaë rhamnoides l.) Of different origin. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2020;75(1):43-48. (In Russ.)
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