Production and stability of a complex prepatation of proteinases by Aspergillus ochraceus L-1 with fibrinolytic and anticoagulant activity
Abstract
A complex preparation was obtained from the culture fluid of micromycete Aspergillus ochraceus L-1 by salting out with ammonium sulfate followed by dialysis and depigmentation to phenolaniline formaldehyde resin at a pH of 8.2 with a specific plasmin-like activity of at least 890 EpNA/mg and a specific activator for protein C activity of less than 130 EpNA/mg was. Comparison of the obtained preparation with caseinolytic, fibrinolytic, fibrinogenolytic and plasminlike activity with commercial analogues terrilithin, trypsin and streptokinase showed its promise for use as a means for thinning purulent burn wounds and fibrin clots. The resulting complex preparation of proteinases had high storage stability at low temperatures for up to 9 months.
About the Authors
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
A. V. Orekhova
Lomonosov Moscow State University; "La Sapienza” University of Rome
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
Piazzale Aldo Moro, 5, Rome, 00185, Italy
E. Conti
2“La Sapienza” University of Rome
Italy
Piazzale Aldo Moro, 5, Rome, 00185, Italy
Italy
Piazzale Aldo Moro, 5, Rome, 00185, Italy
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
N. A. Baranova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
N. S. Egorov
Lomonosov Moscow State University
Russian Federation
Russian Federation
International Biotechnological Center
Leninskie gory 1–12, Moscow, 119234
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Review
For citations:
Osmolovskiy A.A., Orekhova A.V., Conti E., Kreyer V.G., Baranova N.A., Egorov N.S. Production and stability of a complex prepatation of proteinases by Aspergillus ochraceus L-1 with fibrinolytic and anticoagulant activity. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2020;75(3):158-163. (In Russ.)
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