Клетки китайского хомячка в биотехнологических и геронтологических исследованиях
Аннотация
Доступно обновление статьи
Одним из наиболее часто используемых в геронтологии модельных объектов являются дрожжи, в первую очередь – Saccharomyces cerevisiae. Накоплено значительное количество данных, позволяющих считать, что в претерпевающих хронологическое, или «стационарное», старение дрожжах возникают нарушения, сходные с возрастными нарушениями в клетках многоклеточного организма. Однако дрожжи, как и любые объекты исследований, не лишены недостатков – в частности, они, хотя и являются эукариотами, в эволюционном плане отстоят далеко от млекопитающих, что накладывает ограничение на изучение у дрожжей неконсервативных метаболических путей. В некоторых случаях в экспериментах с хронологической моделью лучше использовать клетки млекопитающих – например, клетки китайского хомячка. Они широко используются в промышленности для получения моноклональных антител и рекомбинантных белков. Значительная доля этих продуктов образуется после остановки пролиферации, которая инициирует хронологическое старение культуры. Накопленные данные об особенностях метаболизма клеток, роста культуры и продолжительности ее функциональной жизни являются крайне ценными для геронтологов. Обмен информацией между двумя этими направлениями – биотехнологическим и геронтологическим – будет полезен обеим сторонам.
Ключевые слова
клеточное старение,
клеточные культуры,
«стационарное старение»,
хронологическое старение,
метаболизм,
лактат,
эффект Варбурга
При поддержке: Работа выполнена в рамках государственного задания МГУ, ч. 2 (фундаментальные научные исследования, № АААА-А16-116021660098-8).
Об авторе
Г. В. Моргунова
Сектор эволюционной цитогеронтологии, биологический факультет, Московский государственный университет имени М.В. Ломоносова
Россия
Россия
Моргунова Галина Васильевна – канд. биол. наук, науч. сотр. сектора эволюционной цитогеронтологии биологического факультета МГУ
119234, г. Москва, Ленинские горы, д. 1, стр. 12
Тел.: 8-495-939-15-90
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Рецензия
Для цитирования:
Моргунова Г.В. Клетки китайского хомячка в биотехнологических и геронтологических исследованиях. Вестник Московского университета. Серия 16. Биология. 2020;75(4):237-243.
For citation:
Morgunova G.V. Chinese hamster cells in biotechnological and gerontological research. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2020;75(4):237-243. (In Russ.)
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