Role of secondary oxidative stress in the bactericidal action of antibiotics
Abstract
Exposure to β-lactam, fluoroquinolone and aminoglycoside antibiotics caused an increase in the production of hydrogen peroxide and the expression of OxyR-regulon of the oxidative stress response in Escherichia coli cells. Under the conditions of microaeration, the attenuation of secondary oxidative stress due to the addition of an antioxidant thiourea affected the antibacterial effect of fluoroquinolones. Thiourea potentiated the effect of sub-lethal (which did not reduce the number of colony-forming units below 103/mL) doses of the antibiotic and increased the viability of cells exposed to lethal doses. The addition of thiourea reduced the expression of OxyR-regulon, increased by the sub-lethal antibiotic action, to an antibiotic-free culture level. When exposed to lethal doses, a decrease in the antibiotic-mediated expression of oxidative stress response genes in the presence of thiourea was also observed, however, the expression level remained higher as compared to an antibiotic-free culture. This may indicate the dual role of ROS under antibiotic treatment as the damaging agents contributing to killing and the signal molecules activating stress responses.
About the Authors
A. V. Akhova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch of the Russian Academy of Sciences
Russian Federation
Golev st. 13, Perm, 614081
Russian Federation
Golev st. 13, Perm, 614081
A. G. Tkachenko
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch of the Russian Academy of Sciences
Russian Federation
Golev st. 13, Perm, 614081
Russian Federation
Golev st. 13, Perm, 614081
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Review
For citations:
Akhova A.V., Tkachenko A.G. Role of secondary oxidative stress in the bactericidal action of antibiotics. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2020;75(4):258-264. (In Russ.)
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