Study of cellulose-destroying activity of actinobacteria associated with ants
Abstract
Among actinobacteria associated with various species of ants, their nests and intact soils, cellulolytic activity was screened on a medium with carboxymethyl cellulose using Congo red dye. Assessment of culture broses of tested strains on the CMC-plates and comparing them with titred solutions of the commercial cellulase preparation allowed to quantify their activity. This data were confirmed by the method of evaluating endo-1,4-gluconase activity according to the DNS method. The three most active strains of actinobacteria were isolated from the ants Camponotus vagus, Lasius niger, and Formica cunicularia and belong to the genus Streptomyces. The 16S rDNA sequences of the Streptomyces sp. Fu2, Streptomyces sp. Ln9, and Streptomyces sp. Pe3 strains were deposited in the GenBank under the numbers MN227506, MN227508, and MG 705182 respectively. Specific endoglucanase and β-glucosidase activity of this strains corresponds to that of celluloseand lignin-destroying streptomycetes described in the literature.
About the Authors
Yu. V. Zakalyukina
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Soil Biology, School of Soil Science
Leninskiye gory 1–12, Moscow, 119234
A. R. Zaytsev
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Soil Biology, School of Soil Science
Leninskiye gory 1–12, Moscow, 119234
M. V. Biryukov
Lomonosov Moscow State University;
G.F. Gause Institute of New Antibiotics, Russian Academy of Sciences
Russian Federation
Russian Federation
Department of Microbiology, School of Biology, MSU
Leninskiye gory 1–12, Moscow, 119234;
Bol'shaya Pirogovskaya ul. 11, Moscow, 119021
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Review
For citations:
Zakalyukina Yu.V., Zaytsev A.R., Biryukov M.V. Study of cellulose-destroying activity of actinobacteria associated with ants. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2021;76(1):24-32. (In Russ.)
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