Thermoinactivation kinetics of strain А/BANGKOK/1/1979(Н3N2) influenza virus in the presence of polyallylamine

Currently, an increasing place in antiviral therapy given to various polyelectrolytes. For polyelectrolyte polyallylamine with a molecular weight of 6000 Da, antiviral activity shown against influenza viruses, measles, herpes simplex type 1 and cytomegalovirus. A non-toxic concentration of 30 was determined, at which the antiviral effect for measles and flu is preserved, but there is no toxic effect on cells. At the same time, it known that the simultaneous effect of physical environmental factors and chemical compounds on the virus contributes to a more pronounced decrease in the infectivity of the virus. One of these physical factors is the temperature, the effect of which on the virus leads to its inactivation. The study of thermal inactivation parameters is of great practical importance in the development of influenza vaccines, as well as for studying the decrease in the infectious activity of viral particles when they settle on various surfaces. In this connection, it is of particular interest to study the kinetic and thermodynamic characteristics of the process of thermal inactivation of the influenza virus in the presence of the antiviral compound polyallylamine. In this paper, we have shown that the process of thermal inactivation of the influenza virus in the presence of polyallylamine in the temperature range 38–60°C described by the first-order reaction kinetics. Thermodynamic parameters of thermal inactivation indicate the involvement of surface proteins of the influenza virus in the process of inactivation as result of the interaction of polyallylamine with them. The results obtained indicate the possibility of using polyallylamine as a compound that accelerates the process of thermal inactivation of the influenza virus. 

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