IMPACT OF GLYPROLINES ON CYTOKINES GENE EXPRESSION IN STRESS- AND ACETATE-INDUCED EROSION OF STOMACH
https://doi.org/10.1234/XXXX-XXXX-2014-4-3-8
Abstract
The influence glyprolines PGP and N-acetyl-PGP on gene expression of cytokines in stress-in-duced and acetate-induced ulceration in rats as investigated. Determination of the activity of mRNA in peripheral blood mononuclear cells was performed using methods of reverse transcription and polymerase chain reaction. Intranasal peptide PGP (3,7 mmol/kg) and has a strong protective effect in stress model (59,4%) and acetate model (78,5%) in rats. N-acetyl-PGP does not reduce the area of stress damage in gastric mucosa and has tendency to prevent the development of acetate ulcers. It is shown that in the control animals the formation of stress damage in the gastric mucosa in some cases accompanied by increased transcription of TNFa and inhibition of transcription of IL-4. Development acetate ulcers accompanied by a decrease of gene expression of few cytokines: IFNa, IFNg, IL-2, IL-4, IL-6, IL-12 and TNFa. Protective antiulcer effect PGP followed by increased transcription of IL-6. In group of N-acetyl-PGP is an increase of expression of cytokine genes Th-1 and 2 type IFNa, IFNg and IL-4.
About the Authors
A. D. SangadzhievaRussian Federation
Z. V. Bakaeva
ГБОУ ВПО РНИМУ им. Н.И. Пирогова Минздрава России
Russian Federation
Russian Federation
G. E. Samonina
Russian Federation
M. V. Mezentseva
ФГБУ НИИ вирусологии им. Д.И. Ивановского Минздрава России.
Russian Federation
Russian Federation
L. A. Andreeva
Институт молекулярной генетики РАН
Russian Federation
Russian Federation
N. F. Myasoedov
Институт молекулярной генетики РАН
Russian Federation
Russian Federation
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Review
For citations:
Sangadzhieva A.D., Bakaeva Z.V., Samonina G.E., Mezentseva M.V., Andreeva L.A., Myasoedov N.F. IMPACT OF GLYPROLINES ON CYTOKINES GENE EXPRESSION IN STRESS- AND ACETATE-INDUCED EROSION OF STOMACH. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2014;(4):3-8. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2014-4-3-8
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