Inhibition of ascorbate autoxidation by new strains of lactic acid bacteria
Abstract
Sodium ascorbate is a strong reducing agent able to participate in non-enzymatic reactions with reactive oxygen species. The research work was dedicated to screening of lactic acid bacteria (LAB) strains from the Collection of Microorganisms of the Department of Microbiology (Faculty of Biology, Lomonosov Moscow State University) and to selection of the most promising strains belonged to genera Lactobacillus and Lactococcus. We have investigated the ability to inhibit the ascorbate autoxidation by cells of 16 new strains of LAB from the Collection of Microorganisms of the Department of Microbiology, Lomonosov Moscow State University (CM MSU). Lactobacillus plantarum CM MSU 161 (42.9%), Lactobacillus plantarum CM MSU 520 (36.6%), Lactobacillus plantarum CM MSU 508 (33%) and Lactobacillus paracasei CM MSU 527 (38.1%) had the greatest inhibitory effect on ascorbate autoxidation. Lactobacillus acidophilus CM MSU 146, Lactobacillus plantarum CM MSU 162, Lacticaseibacillus rhamnosus CM MSU 529, Lactobacillus paracasei CM MSU 544, Lactobacillus caucasicus CM MSU 155, Lactococcus lactis ssp. lactis CM MSU 170 had no detectable effect on inhibition of ascorbate autoxidation. These strains were compared with strains from other collections. In particular, the majority of Lactobacillus plantarum strains as well as Lactobacillus brevis CM MSU 535 (20.7%) from the CM MSU demonstrated a higher percentage of inhibition of ascorbate autoxidation compared with the strains from the ATCC and the DSMZ collections. We have shown that this method can be used as an express testing to reveal the general ability of lactic acid bacterial cells to resist reactive oxygen species and to assess their antioxidant status. Thus, the ability to inhibit autooxidation of ascorbate can be used as one of the potential positive factors for selection of probiotics among LAB.
Keywords
Lactobacillus,
lactic acid bacteria (LAB),
probiotics,
antioxidants,
ascorbate autoxidation,
vitamin C
Supporting agencies: The work was carried out within the framework of the state task on the topic of the Department of Microbiology of MSU “Physiology and Biochemistry of Phototrophic and Chemotrophic Microorganisms” (CITIS number 121032300094-7).
About the Authors
A. I. Klimko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Microbiology Department, Faculty of Biology
Leninskiye Gory 1–12, Moscow, 119234
T. A. Cherdyntseva
Lomonosov Moscow State University
Russian Federation
Russian Federation
Microbiology Department, Faculty of Biology
Leninskiye Gory 1–12, Moscow, 119234
A. I. Netrusov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Microbiology Department, Faculty of Biology
Leninskiye Gory 1–12, Moscow, 119234
A. L. Bryukhanov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Microbiology Department, Faculty of Biology
Leninskiye Gory 1–12, Moscow, 119234
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Review
For citations:
Klimko A.I., Cherdyntseva T.A., Netrusov A.I., Bryukhanov A.L. Inhibition of ascorbate autoxidation by new strains of lactic acid bacteria. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2021;76(4):278-282. (In Russ.)
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