Morphological and molecular genetic analysis of green microalgal strains isolated from commercial biological products based on “living Сhlorella”
Abstract
In the present study, Chlorella-like strains of green microalgae from various commercial biological products were studied for the first time using morphological and molecular genetic analyses. It was found that representatives of the Chlorella clade or sister Parachlorella clade were present in all analyzed samples. In addition, microalgae from the genera Edaphochlorella, Chloroidium, Edaphochloris and Muriella belonging to the Trebouxiophyceae class, as well as Coelastrella and Chromochloris belonging to the Chlorophyceae class were found. According to direct light microscopy data, filamentous cyanobacteria were observed in all preparations, and the presence of diatoms was also noted in the biopreparation “Gera”. Since the correct identification of green microalgae based on phenotypic traits is very difficult due to their scarcity, lack of information and variability, the control of the composition of microalgae in biological products should be carried out using modern molecular genetic methods.
Keywords
biological products,
Chlorella-like algae,
morphology,
phylogeny,
identification,
species richness,
cryptic species
Supporting agencies: The study was funded by the Russian Foundation for Basic Research, project number 19-34-60002.
About the Authors
E. S. Krivina
Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”
Russian Federation
Russian Federation
prosp. Nauki 3, Pushchino, 142290
A. D. Temraleeva
Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”
Russian Federation
Russian Federation
prosp. Nauki 3, Pushchino, 142290
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Krivina E.S., Temraleeva A.D. Morphological and molecular genetic analysis of green microalgal strains isolated from commercial biological products based on “living Сhlorella”. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2022;77(3):160-165. (In Russ.)
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