Comparison of the in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus and contained in snake venom
https://doi.org/10.55959/MSU0137-0952-16-78-1-6
Abstract
The in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus L-1 and contained in snakes’ venoms (Protac® and RVV-X® preparations) was studied. The severity of the action of micromycete protease in relation to plasmas of humans and warm-blooded animals, as well as human plasmas deficient in certain factors of the hemostasis system, in comparison with snake proteases in reactions with chromogenic peptide substrates of activated protein C and factor X, as well as using the activated partial thromboplastin time (APTT) test. The optimal time of preincubation of the micromycete A. ochraceus L-1 protease with human blood plasma (3 min) and the concentration of the chromogenic peptide substrate of activated protein C (from 0.1 to 0.5 mg/ml) necessary for the correct determination of protein C with her help.
Keywords
proteases of snake venom,
Aspergillus ochraceus protease,
anticoagulant enzymes,
hemostasis protein activators,
chromogenic peptide substrates,
APTT
Supporting agencies: This work was supported by the Financial Council for Grants of the President of the Russian Federation No. SP-3906.2021.4
About the Authors
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
I. Dombeck
University of Hohenheim
Germany
Germany
Faculty of Natural Sciences
Garbenstraße 13, Stuttgart, 70599
S. Hauke
Technical University of Munich
Germany
Germany
School of Engineering and Design
Boltzmann str. 15, Garching, 85748
A. V. Orekhova
Lomonosov Moscow State University;
Federal State Budgetary Institution “National Medical Research Center for Therapy and Preventive Medicine” of the Ministry of Health of Russia
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University
Leninskie gory 1–12, Moscow, 119234,
Petroverigsky per., 10, Moscow, 101990
V. G. Kreyer
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
Leninskie gory 1–12, Moscow, 119234
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Review
For citations:
Osmolovskiy A.A., Dombeck I., Hauke S., Orekhova A.V., Kreyer V.G. Comparison of the in vitro anticoagulant action of proteases secreted by the micromycete Aspergillus ochraceus and contained in snake venom. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2023;78(1):43-48. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-78-1-6
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