Исследование противовоспалительных свойств секреторно-экскреторных продуктов плероцеркоидов лентеца чаечного Dibothriocephalus dendriticus и лигулы Ligula interrupta

В данной работе впервые был исследован противовоспалительный потенциал секреторно-экскреторных продуктов (СЭП) плероцеркоидов лентеца чаечного Dibothriocephalus dendriticus и лигулы Ligula interrupta в модели индуцированной липополисахаридом активации макрофагов in vitro. В качестве модели макрофагов была использована клеточная линия моноцитов, полученная от пациента с острым моноцитарным лейкозом, THP-1. Противовоспалительные свойства СЭП определяли по содержанию в инкубационной среде цитокинов – фактора некроза опухоли и интерлейкина-6 – с помощью коммерческих наборов для иммуноферментного анализа. Результаты проведенного нами исследования свидетельствуют о том, что СЭП плероцеркоидов L. interrupta обладают выраженным противовоспалительным эффектом, в то время как СЭП плероцеркоидов D. dendriticus таким эффектом не обладают. Далее мы исследовали противовоспалительные свойства СЭП L. interrupta в модели каррагинан-индуцированного воспаления в «воздушном мешке» у мышей. Было обнаружено значимое уменьшение объема воспалительного экссудата под действием СЭП L. interrupta, а также увеличение уровня цитокина интерлейкина-6. В то же время СЭП L. interrupta не оказывали влияния на число клеток в 1 мл экссудата, а также на уровень противовоспалительного цитокина – фактора некроза опухоли. Низкомолекулярная фракция СЭП L. interrupta также увеличивала уровень противовоспалительного цитокина интерлейкина-10, что свидетельствует о более выраженном противовоспалительном эффекте по сравнению с высокомолекулярной фракцией. Полученные результаты, в целом, свидетельствуют о противовоспалительных свойствах СЭП плероцеркоидов L. interrupta. Однако механизм противовоспалительного действия не выяснен и требует дальнейшего исследования.

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