Cryo-electron microscopy of enveloped viruses using upgraded transmission electron microscope: Influenza type A, B viruses and SARS-CoV-2
https://doi.org/10.55959/MSU0137-0952-16-78-3S-4
Abstract
Cryo-electron microscopy (cryo-EM) is indispensable for the structural studies of enveloped viruses – dangerous pathogens of humans and animals. Yet, it requires highly specialized equipment as well as careful sample preparation. In this work, the capabilities of transmission electron microscope JEOL JEM-2100 equipped with cryo-transfer holder are used, and preliminary cryo-EM data for influenza A and B virus strains and SARS-CoV-2 inactivated with beta-propiolactone are presented. Image analysis allows: (1) to distinguish “empty” viral particles from “full” ones (containing nucleocapsid); (2) to visualize the lipid bilayer of the viral envelope; (3) identify influenza virus surface antigens and the M1 protein layer combined with the inner lipid monolayer; (4) distinguish different morphology of S-spikes on the surface of inactivated SARS-CoV-2 virions. The developed approach provides good image quality for both fundamental and applied research.
Keywords
influenza A and B viruses,
hemagglutinin,
matrix M1 protein,
SARS-CoV-2,
S-protein,
beta-propiolactone,
cryo-EM
Supporting agencies: The research was funded by Russian Foundation for Basic Research, project number 20-54-14006.
About the Authors
L. V. Kordyukova
Belozersky Institite of Physico-Chemucal Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie Gory, 119991, Moscow
A. V. Moiseenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Faculty of Biology
1–32 Leninskie Gory, 119234, Moscow
T. A. Timofeeva
D.I. Ivanovsky Institute of Virology, N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
16 Gamaleya st., 123098, Moscow
I. T. Fedyakina
D.I. Ivanovsky Institute of Virology, N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
16 Gamaleya st., 123098, Moscow
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Review
For citations:
Kordyukova L.V., Moiseenko A.V., Timofeeva T.A., Fedyakina I.T. Cryo-electron microscopy of enveloped viruses using upgraded transmission electron microscope: Influenza type A, B viruses and SARS-CoV-2. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2023;78(3S):21-26. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-78-3S-4
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