Cryo-EM structure of bovine chaperonin TRiC/CCT in open conformation
T. B. Stanishneva-Konovalova,
E. B. Pichkur,
S. S. Kudryavtseva,
I. A. Yaroshevich,
A. N. Semenov,
E. G. Maksimov,
A. V. Moiseenko,
O. I. Volokh,
V. I. Muronetz https://doi.org/10.55959/MSU0137-0952-16-78-3S-7
Abstract
In this work, conditions were selected for obtaining a sample of eukaryotic chaperonin TRiC suitable for studying by cryo-electron microscopy. Using the method of differential scanning (time-resolved) fluorimetry, the temperature stability of protein samples at different concentrations of salt and glycerol was compared, and then the selected conditions were used to prepare the sample for microscopy. As a result, the structure of bovine TRiC in an open conformation was obtained at 4.42 Å resolution.
Keywords
chaperonin,
TRiC,
CCT,
cryo-electron microscopy,
quaternary protein structure,
differential scanning (time-resolved) fluorimetry
Supporting agencies: The research was funded by the Russian Science Foundation, project number 19-74-20055.
About the Authors
T. B. Stanishneva-Konovalova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology
1–12 Leninskie Gory, 119234, Moscow
E. B. Pichkur
National Research Centre “Kurchatov institute”
Russian Federation
Russian Federation
Department of Structural Biology
1 Kurchatov Square, 123182, Moscow
S. S. Kudryavtseva
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie Gory, 119991, Moscow
I. A. Yaroshevich
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Biophysics, Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology
1–12 Leninskie Gory, 119234, Moscow
A. N. Semenov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology
1–12 Leninskie Gory, 119234, Moscow
E. G. Maksimov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology
1–12 Leninskie Gory, 119234, Moscow
A. V. Moiseenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Electron Microscopy Laboratory, Faculty of Biology
1–32 Leninskie Gory, 119234, Moscow
O. I. Volokh
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, Faculty of Biology
1–12 Leninskie Gory, 119234, Moscow
V. I. Muronetz
Lomonosov Moscow State University
Russian Federation
Russian Federation
Belozersky Institute of Physico-Chemical Biology
1–40 Leninskie Gory, 119991, Moscow
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Review
For citations:
Stanishneva-Konovalova T.B., Pichkur E.B., Kudryavtseva S.S., Yaroshevich I.A., Semenov A.N., Maksimov E.G., Moiseenko A.V., Volokh O.I., Muronetz V.I. Cryo-EM structure of bovine chaperonin TRiC/CCT in open conformation. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2023;78(3S):40-46. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-78-3S-7
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