Cryo-electron microscopy at the Faculty of Biology of Lomonosov Moscow State University
https://doi.org/10.55959/MSU0137-0952-16-78-3S-9
Abstract
This paper demonstrates an example of a successful upgrade of a JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope designed for routine tasks of sample preparation and quality evaluation. As a result of the upgrade, the instrument allows the subnanometer resolution of protein molecule reconstructions (within 8 Å). The influence of graphene and amorphous carbon support films to prevent the effect of preferred orientation of protein particles in the frozen sample is discussed.
Keywords
Cryo-electron microscopy,
structural biology,
single particle analysis,
equipment upgrading,
structure,
virus,
nucleosome
Supporting agencies: The modernization of the microscope was carried out as part of a project of the Ministry of Science and Higher Education of the Russian Federation. The analysis of samples and the acquisition of three-dimensional structures of the tick-borne encephalitis virus were performed with financial support from the Development Program of Moscow State University, project number 23A-SH04-01. The development of nucleosome measurement techniques was conducted with the support of the Russian Science Foundation, project number 19-74-30003.
About the Authors
A. V. Moiseenko
Lomonosov Moscow State University
Russian Federation
Russian Federation
Electron Microscopy Laboratory, Faculty of Biology
1–32 Leninskie Gory, 119234, Moscow
A. M. Egorov
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Chemical Enzymology, Faculty of Chemistry
1–11 Leninskie Gory, 119234, Moscow
K. V. Shaitan
Lomonosov Moscow State University
Russian Federation
Russian Federation
Faculty of Biology
1–73 Leninskie Gory, 119234, Moscow
O. S. Sokolova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Faculty of Biology
1–73 Leninskie Gory, 119234, Moscow
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Review
For citations:
Moiseenko A.V., Egorov A.M., Shaitan K.V., Sokolova O.S. Cryo-electron microscopy at the Faculty of Biology of Lomonosov Moscow State University. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2023;78(3S):51-56. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-78-3S-9
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