Взаимодействующие гены PARG1 и EXA1 являются важными регуляторами аутоиммунитета у растений
https://doi.org/10.55959/MSU0137-0952-16-79-4-8
Аннотация
Исследования выполнены на растениях двойного мутанта резуховидки (Arabidopsis thaliana) parg1 exa1, характеризующегося смертью клеток по краю листовой пластинки, активацией экспрессии генов иммунных рецепторов, генов PR-белков и генов гиперчувствительного ответа, что является признаком аутоиммунитета. В то же время, в отличие от других мутантов c проявлением аутоиммунитета, мутант parg1 exa1 демонстрировал приобретение клетками листа плюрипотентности и способность к регенерации нового края листа. В связи с необычным фенотипом в данной работе проведен углубленный анализ транскриптома молодых листьев мутанта и растений дикого типа. У мутанта обнаружено повышение уровня экспрессии всех основных компонентов иммунного ответа, включая гены Са2+-проводящих каналов, НАДФН-оксидаз, генов синтеза салициловой кислоты, жасмоновой кислоты и многие другие. Полученные данные свидетельствуют о том, что гены PARG1 и EXA1, контролирующие уровень поли(АДФ-рибозилирования) и процесс нонсенс-опосредованной деградации РНК соответственно, являются важными регуляторами иммунного ответа. Их функция необходима для предотвращения чрезмерной активации защитных систем в растениях.
Ключевые слова
аутоиммунитет растений,
транскриптом,
рецепторы иммунитета,
салициловая кислота,
клеточная смерть,
мутации
При поддержке: Работа выполнена при финансовой поддержке Российского фонда фундаментальных исследований (проект №19-04-00149_a).
Об авторах
Е. В. Куприянова
Кафедра генетики, биологический факультет, Московский государственный университет имени М.В. Ломоносова
Россия
Россия
Куприянова Евгения Владимировна – канд. биол. наук, ст. науч. сотр. кафедры
119234, г. Москва, Ленинские горы, д. 1, стр. 12, Тел.: 8-495-939-54-90
К. А. Манахова
Центр генетики и наук о жизни, Научно-технологический университет Сириус
Россия
Россия
Манахова Карина Айратовна – науч. сотр. (направление «Генетика») научного центра
354340, Краснодарский край, пгт Сириус, Тел.: 8-800-100-41-55
Т. А. Ежова
Кафедра генетики, биологический факультет, Московский государственный университет имени М.В. Ломоносова
Россия
Россия
Ежова Татьяна Анатольевна – докт. биол. наук, проф. кафедры
119234, г. Москва, Ленинские горы, д. 1, стр. 12, Тел.: 8-495-939-54-90
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Рецензия
Для цитирования:
Куприянова Е.В., Манахова К.А., Ежова Т.А. Взаимодействующие гены PARG1 и EXA1 являются важными регуляторами аутоиммунитета у растений. Вестник Московского университета. Серия 16. Биология. 2024;79(4):287-297. https://doi.org/10.55959/MSU0137-0952-16-79-4-8
For citation:
Kupriyanova E.V., Manakhova K.A., Ezhova T.A. PARG1 and EXA1 interacting genes are important regulators of autoimmunity in plants. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2024;79(4):287-297. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-79-4-8
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