The morphology of erythrocytes reveals differences in the mechanisms of erythrocyte derived extracellular vesicle formation
https://doi.org/10.55959/MSU0137-0952-16-80-3S-4
Abstract
Erythrocyte-derived extracellular vesicles (EDEVs) are a promising tool for “targeted drug delivery,” and this study aimed to compare several methods of obtaining EDEVs from erythrocytes in vitro. Scanning electron microscopy revealed that under certain treatments (calcium ionophore A23187, SDS, LPA, and incubation at 50°C), erythrocytes shed EDEVs. The differences in the morphology of erythrocytes subjected to EDEVs-stimulating treatments suggest distinct mechanisms of EDEVs formation. Raman spectroscopy showed that EDEVs obtained by heat treatment may contain hemoglobin, while SDS-induced treatment produces hemoglobin-free vesicles. The data obtained will allow for a targeted selection of EDEVs production methods based on their required composition.
Supporting agencies: This research was funded by Russian Science Foundation, project number 23-74-00006.
About the Authors
E. Yu. Parshina
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–24 Leninskie Gory, Moscow, 119234
L. S. Frolova
Department of Chemistry, Irving K. Barber Faculty of Science, University of British Columbia Okanagan
Canada
Canada
3247 Research Road, Kelowna, BC V1V 1V7
G. V. Maximov
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–24 Leninskie Gory, Moscow, 119234
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Review
For citations:
Parshina E.Yu., Frolova L.S., Maximov G.V. The morphology of erythrocytes reveals differences in the mechanisms of erythrocyte derived extracellular vesicle formation. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3S):23-30. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-4
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