Visualization of artificial polynucleosomal constructs with atomic force microscopy
https://doi.org/10.55959/MSU0137-0952-16-80-3S-8
Abstract
Genetic material of the cell in interphase nucleus is present in a form of a dense DNA-protein structure named chromatin. Structure and dynamics of single nucleosome, which is the basic unit of DNA compactization, is currently well-studied, although the data about the structural and functional organization of higher-level chromatin folding, is still scarce. In the present work, a method of visualization of polynucleosomal constructs using atomic force microscopy is proposed. Polynucleosome assembly on a plasmid with the use of recombinant histone octamers was demonstrated. It was established that glutaraldehyde treatment of polynucleosome sample before its immobilization on a surface preserves nucleosomes, and their height and width corresponds well with previously obtained data. Plasmids themselves were predominantly In extended conformation, which may help studying DNA-protein interactions.
Supporting agencies: The research was funded by Russian Science Foundation, project no. 21-64-00001P.
About the Authors
A. V. Lyubitelev
Bioengineering Department, School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskiye Gory, Moscow, 119234
D. V. Bagrov
Bioengineering Department, School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskiye Gory, Moscow, 119234
O. V. Geraskina
Institute of Gene Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
34/5 Vavilov Str., Moscow, 119334
V. M. Studitsky
Bioengineering Department, School of Biology, Lomonosov Moscow State University; Fox Chase Cancer Center
Russian Federation
Russian Federation
1–12 Leninskiye Gory, Moscow, 119234
333 Cottman Ave., Philadelphia, Pennsylvania, 19111
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Review
For citations:
Lyubitelev A.V., Bagrov D.V., Geraskina O.V., Studitsky V.M. Visualization of artificial polynucleosomal constructs with atomic force microscopy. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3S):52-58. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-8
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