PARP1-dependent alterations in nucleosome organization: potential involvement of p53
https://doi.org/10.55959/MSU0137-0952-16-80-3S-10
Abstract
Chromatin of eukaryotic organisms is a complexly organized and dynamic complex. Chromatin proteins provide proper regulation of gene expression, DNA replication, and DNA repair. Among the most important regulators of chromatin architecture among non-histone proteins are p53 and PARP1, which are involved in the cellular response to DNA damage. In the present study, we investigated the cooperative and competitive binding of the DNA-binding domain (DBD) of p53 and the enzyme PARP1 to mononucleosomes reconstituted on the Widom 603 sequence with an embedded p53 binding site. To detect interactions, the electrophoretic mobility shift assay (EMSA) method with fluorescently labeled DNA. Complexes were formed in two ways: nucleosomes were pre-incubated with p53 DBD and then PARP1 was added, or the nucleosome–PARP1 complex was formed first and then p53 DBD was introduced. The results showed that the order of protein addition determines the nature of their interaction with the nucleosome: at low p53 concentrations, displacement of this protein by PARP1 is observed, while at higher p53 concentrations, stable nucleosome–p53 complexes are formed, undisturbed by PARP1. No stable ternary nucleosome–p53–PARP1 complexes were detected.
Supporting agencies: This research was supported by the Russian Science Foundation (project no. 21-64- 00001-P).
About the Authors
D. O. Koshkina
School of Biology, Lomonosov Moscow State University; Institute of Gene Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
1–12 Leninskie gory, Moscow, 119234
34/5 Vavilov Str., Moscow, 119334
N. V. Maluchenko
School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie gory, Moscow, 119234
A. M. Novichkova
School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie gory, Moscow, 119234
A. V. Feofanov
School of Biology, Lomonosov Moscow State University; Institute of Gene Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
1–12 Leninskie gory, Moscow, 119234
34/5 Vavilov Str., Moscow, 119334
V. M. Studitsky
School of Biology, Lomonosov Moscow State University; Fox Chase Cancer Center
Russian Federation
Russian Federation
1–12 Leninskie gory, Moscow, 119234
333 Cottman Ave., Philadelphia, 19111, Pennsylvania
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Koshkina D.O., Maluchenko N.V., Novichkova A.M., Feofanov A.V., Studitsky V.M. PARP1-dependent alterations in nucleosome organization: potential involvement of p53. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3):67-72. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-10
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