Structure of the translation pre-initiation complex from a plant extract
https://doi.org/10.55959/MSU0137-0952-16-80-3S-14
Abstract
Translation pre-initiation complexes are typical components of the cytoplasm of eukaryotic cells, consisting of 40S ribosomal subunits bound to initiation factors. Actively studied in mammals and protozoa, these complexes are involved in the initiation of mRNA translation. We studied the abundance and structure of plant pre-initiation complexes using single particle cryo EM analysis of wheat germ extract preparations. It was found that about 29% of free 40S subunits form a complex with the initiation factors eIF3 and eIF1A. The structure of the complex was reconstructed with overall resolution better than 3 Å and the core of factor eIF3 with a resolution of 3.6 Å. These are the first structural data on the structure of initiation ribosomal complex in plants. We constructed the atomic models of the iIF3 core and distal subunits which revealed notable differences from those in mammalian cells.
Supporting agencies: The research was funded by Russian Foundation for Basic Research, project no. 19-74 20186.
About the Authors
O. V. Kravchenko
Institute of Protein Research, Russian Academy of Sciences
Russian Federation
Russian Federation
4 Institutskaya Str., Pushchino, Moscow Region, 142290
Y. M. Chesnokov
National Research Center, “Kurchatov Institute”
Russian Federation
Russian Federation
1 I.V. Kurchatov Sq., Moscow, 123182
Zh. A. Afonina
Institute of Protein Research, Russian Academy of Sciences
Russian Federation
Russian Federation
4 Institutskaya Str., Pushchino, Moscow Region, 142290
K. S. Vassilenko
Institute of Protein Research, Russian Academy of Sciences
Russian Federation
Russian Federation
4 Institutskaya Str., Pushchino, Moscow Region, 142290
References
1. Hashem Y., Des Georges A., Dhote V., Langlois R., Liao H., Grassucci R. A., Frank J. Structure of the mamma lian ribosomal 43S preinitiation complex bound to the scan ning factor DHX29. Cell. 2013;153(5):1108–1119.
2. Des Georges A., Dhote V., Kuhn, L., Hellen C.U., Pestova T.V., Frank J., Hashem Y. Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Nature. 2015;525(7570):491–495.
3. Eliseev B., Yeramala L., Leitner A., Karup pasamy M., Raimondeau E., Huard K., Alkalaeva E., Ae bersold R., Schaffitzel, C. Structure of a human cap-de pendent 48S translation pre-initiation complex. Nucleic Acids Res. 2018;46(5):2678–2689.
4. Brito Querido J., Sokabe M., Kraatz S., Gordi yenko Y., Skehel J.M., Fraser C.S., Ramakrishnan V. Struc ture of a human 48 S translational initiation complex. Sci ence. 2020;369(6508):1220–1227.
5. Heuer A., Gerovac M., Schmidt C., Trowitzsch S., Preis A., Kötter P., Berninghausen O., Becker T., Beckmann R., Tampe R. Structure of the 40S–ABCE1 post-splitting complex in ribosome recycling and translation initiation. Nat. Struct. Mol. Biol. 2017;24(5):453–460.
6. Kratzat H., Mackens-Kiani T., Ameismeier M., Potocnjak M., Cheng J., Dacheux E., Namane A., Bern inghausen O., Herzog F., Fromont-Racine M., Becker T., Beckmann R. A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes. EMBO J. 2021;40(1):e105179.
7. Shirokov V.A., Kommer A., Kolb V.A., Spirin A.S. Continuous-exchange protein-synthesizing systems. Methods in Molecular Biology: In Vitro Transcription and Translation Protocols. 2nd edn, Vol. 375. Ed. G. Grandi. N.J.: Humana; 2007:19–55.
8. Kravchenko O.V., Baymukhametov T.N., Afo nina Z.A., Vassilenko K.S. High-resolution structure and in ternal mobility of a plant 40S ribosomal subunit. Int. J. Mol. Sci. 2023;24(24):17453.
9. Petrychenko V., Yi S.H., Liedtke D., Peng B.Z., Rodnina M.V., Fischer N. Structural basis for translational control by the human 48S initiation complex. Nat. Struct. Mol. Biol. 2025;32(1):62–72.
10. Brito Querido J., Sokabe M., Díaz-López I., Gordiyenko Y., Fraser C., Ramakrishnan V. The structure of a human translation initiation complex reveals two inde pendent roles for the helicase eIF4A. Nat. Struct. Mol. Biol. 2024;31(3):455–464.
Review
For citations:
Kravchenko O.V., Chesnokov Y.M., Afonina Zh.A., Vassilenko K.S. Structure of the translation pre-initiation complex from a plant extract. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3S):96–100. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-14
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