Cryo-electron microscopy study of the axial fiber of the Stx-converting bacteriophage phi24B
https://doi.org/10.55959/MSU0137-0952-16-80-3S-15
Abstract
Using cryo-electron microscopy, we studied the structure of the axial fiber protein gp56, a component of the adsorption apparatus of the Stx-converting phage phi24B. The axial fiber is highly mobile, and the gp56 trimer is encased by the bulky hexameric nozzle protein gp57, creating a symmetry-mismatch interface that complicates three-dimensional reconstruction. By applying symmetry expansion and local orientation refinement, we generated a density map of the axial fiber, visualized the tertiary structure of its globular domains, and determined their positions relative to the other proteins of the phi24B adsorption apparatus.
Supporting agencies: This work was supported by the Russian Science Foundation, project no. 25-24-00110.
About the Authors
A. V. Moiseenko
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie gory, 119234, Moscow
M. A. Bubenchikov
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie gory, 119234, Moscow
Y. Zh. Zhang
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University; Faculty of Biology, MSU-BIT University
Russian Federation
Russian Federation
1–12 Leninskie gory, 119234, Moscow
1 International University Park Road, Shenzhen, Guangdong Province, 518172
A. S. Kuznetsov
Winogradsky Institute of Microbiology, Federal Research Center “Biotechnology”, Russian Academy of Sciences
Russian Federation
Russian Federation
7–2 60-letiya Oktyabrya Str., Moscow, 117312
A. V Letarov
Winogradsky Institute of Microbiology, Federal Research Center “Biotechnology”, Russian Academy of Sciences
Russian Federation
Russian Federation
7–2 60-letiya Oktyabrya Str., Moscow, 117312
O. S. Sokolova
Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University; Faculty of Biology, MSU-BIT University
Russian Federation
Russian Federation
1–12 Leninskie gory, 119234, Moscow
1 International University Park Road, Shenzhen, Guangdong Province, 518172
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Review
For citations:
Moiseenko A.V., Bubenchikov M.A., Zhang Y.Zh., Kuznetsov A.S., Letarov A.V., Sokolova O.S. Cryo-electron microscopy study of the axial fiber of the Stx-converting bacteriophage phi24B. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3S):101–105. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-15
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