Analysis of heterochromatin mesoscale architecture by cryo-electron tomography
https://doi.org/10.55959/MSU0137-0952-16-80-3S-18
Abstract
Cryo-ET (cryo-electron tomography) is a powerful tool for studying the structure of biological objects in their native state. However, Cryo-ET is still not widely used to study cellular organelles including cell nucleus and chromatin in situ. In our work, we aimed to investigate the possibilities of employing Cryo-ET for studying the architecture of natively preserved chromatin, focusing on the possibility of using a cryoCLEM (cryo-correlative light and electron microscopy) approach to target final steps of sample preparation by focused ion beam (FIB) milling exactly to the area of interest – specifically, heterochromatin loci.
Supporting agencies: This work was supported by the Russian Science Foundation, project no. 23-74-00021.
About the Authors
E. P. Kazakov
Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University; Department of Cell Biology and Histology, School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie gory, Moscow, 119991
1–12 Leninskie gory, Moscow, 119234
Y. M. Chesnokov
Kurchatov Complex of NBICS Nature-Like Technologies, National Research Center “Kurchatov Institute”
Russian Federation
Russian Federation
1 Academician Kurchatov Sq., Moscow, 123182
I. I. Kireev
Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University; Department of Cell Biology and Histology, School of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie gory, Moscow, 119991
1–12 Leninskie gory, Moscow, 119234
S. A. Golyshev
Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie gory, Moscow, 119991
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Review
For citations:
Kazakov E.P., Chesnokov Y.M., Kireev I.I., Golyshev S.A. Analysis of heterochromatin mesoscale architecture by cryo-electron tomography. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3):118–123. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3S-18
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