Comparative study of auditory sensitivity in normal Wistar rats and heterozygous rats with reduced expression f the dopamine transporter (DAT-1) gene
https://doi.org/10.55959/MSU0137-0952-16-80-3-3
Abstract
The comparative electrophysiological study of auditory sensitivity of intact Wistar rats and heterozygous rats of the transgenic DAT-het line with a reduced expression level of the Slc6a3 gene encoding the dopamine reuptake transporter (DAT-1), providing an experimental model of dopaminergic neurons pathology, was performed. The amplitude and time parameters of auditory brainstem responses recorded in rats of both lines under presentation of paired clicks and single tones were analyzed. Generally, the auditory sensitivities of Wistar and DAT-het rats were similar, but a comparative analysis of the amplitudes of auditory brainstem response peaks, obtained from experimental animals, revealed a significantly greater amplitude of peak 1 in DAT-het rats. Thus, the data obtained suggest that DAT-het heterozygotes differ from rats with a normally functioning dopamine reuptake DAT-1 transporter by an increased level of dopaminergic signaling via activation of D1 receptors localized in the membranes of neurons of the cochlea spiral ganglion.
Keywords
rat,
dopaminergic system,
attention deficit hyperactivity disorder,
transgenic animals,
hearing,
auditory brainstem response
Supporting agencies: The research was supported by state budget funds (no. 075-00263-25-00). The work of Z.S. Fesenko and E.V. Efimova was funded by St. Petersburg State University (project no. 117033714).
About the Authors
G. D. Khorunzhii
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Russian Federation
Russian Federation
Torez Ave. 44, St. Petersburg, 194223
M. A. Egorova
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Russian Federation
Russian Federation
Torez Ave. 44, St. Petersburg, 194223
Z. S. Fesenko
Saint Petersburg State University, Saint Petersburg State University Institute of Translational Biomedicine
Russian Federation
Russian Federation
Universitetskaya Emb. 7/9, St. Petersburg, 199034
E. V. Efimova
Saint Petersburg State University, Saint Petersburg State University Institute of Translational Biomedicine
Russian Federation
Russian Federation
Universitetskaya Emb. 7/9, St. Petersburg, 199034
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For citations:
Khorunzhii G.D., Egorova M.A., Fesenko Z.S., Efimova E.V. Comparative study of auditory sensitivity in normal Wistar rats and heterozygous rats with reduced expression f the dopamine transporter (DAT-1) gene. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2025;80(3):165-172. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-80-3-3
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