ESTIMATION OF EVOLUTIONAL STABILITY OF INFLUENZA A VIRUS. PREDICTION OF VARIABLE REGIONS IN DOMAIN STRUCTURE OF M1 PROTEIN

Abstract

Influenza virus is one of human pathogens, responsible for several pandemics with high death rate. For statistical analysis of coding regions of genes from extensive sample of Influenza A viruses two programs were written. While inner viral proteins appeared to be evolutionary stable, surface antigens and non structural protein NS1 are highly variable. Using written programs to predict evolutionary variable regions inside M1 protein sequence it was shown that N-domain is the most conservative one while M- and C-domains are more variable. In addition, C-domain was shown to be the most unfolded one according to DISOPRED algorithm prediction and, thus, possesses the most structural plasticity.