NEW PHYTOVIRAL VECTOR FOR SUPEREXPRESSION OF TARGET PROTEINS IN PLANTS
https://doi.org/10.1234/XXXX-XXXX-2013-3-17-22
Abstract
Previously, we obtained a new viral vector, based on the Alternanthera mosaic virus strain MU (AltMV-MU). The gene of interest was placed under control of two subgenomic promoters (sgp). Viral vector provided the superexpression of target proteins in Nicotiana benthamiana. In the present work, to increase the level of protein expression this viral vector was modified and the coat protein gene was placed under control of three sgp. The new viral vector provided superexpression of protein of interest in plants and its amount was more than 50% of the total soluble protein of cells. The comparison of the target protein expression efficiency in plants transformed by viral vectors contained two and three sgp, was done.
About the Authors
E. V. PutlyaevRussian Federation
A. A. Smirnov
Russian Federation
E. A. Lazareva
Russian Federation
G. V. Klink
Russian Federation
O. V. Karpova
Russian Federation
I. G. Atabekov
Russian Federation
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Review
For citations:
Putlyaev E.V., Smirnov A.A., Lazareva E.A., Klink G.V., Karpova O.V., Atabekov I.G. NEW PHYTOVIRAL VECTOR FOR SUPEREXPRESSION OF TARGET PROTEINS IN PLANTS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2013;(3):17-22. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2013-3-17-22
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