ARTIFICIAL SEEDS AS THE METHOD OF THE ECOLOGICAL PURE MEDICINAL RAW MATERIAL PRODUCTION AND THE WAY OF PRESERVATION OF THE RARE PLANTS
https://doi.org/10.1234/XXXX-XXXX-2011-2-4-6
Abstract
Artificial seeds production by the encapsulation of somatic embryos in alginate gel is widely applied method in micropropagation. In this study, artificial seeds were obtained from pRi T-DNA transformed roots (known also as “hairy root culture”) of skullcap (Scutellaria baicalensis Georgi). Root segments in artificial seeds were viable after prolonged storage at +4°C. The main parameters of hairy root cultures, produced from the artificial seeds, were investigated. Using encapsulation method, the hairy root culture of skullcap with higher productivity was obtained. After encapsulation there was observed the chloroplasts formation in root cells under light conditions, and the total amount of chlorophyll and the content of flavonoids in green roots were determined.
About the Authors
M. Yu. Vdovitchenko
Институт физиологии растений им. К.А. Тимирязева РАН, г. Москва
Russian Federation
Russian Federation
I. N. Kuzovkina
Институт физиологии растений им. К.А. Тимирязева РАН, г. Москва
Russian Federation
Russian Federation
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Review
For citations:
Vdovitchenko M.Yu., Kuzovkina I.N. ARTIFICIAL SEEDS AS THE METHOD OF THE ECOLOGICAL PURE MEDICINAL RAW MATERIAL PRODUCTION AND THE WAY OF PRESERVATION OF THE RARE PLANTS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2011;(2):4-6. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2011-2-4-6
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