DNA SEQUENCES LOCATED BEFORE 4.5SH RNA GENE CAN HAVE A GREAT IMPACT ON ITS TRANSCRIPTION BY RNA POLYMERASE III IN VITRO AND IN THE WHOLE CELL
https://doi.org/10.1234/XXXX-XXXX-2011-2-16-18
Abstract
4,5SH RNA genes contain an internal RNA polymerase III promoter consisting of A and B boxes. To analyze the influence of the various sequences inserted upstream from 4,5SH RNA gene we used in vitro transcription and transfection in the whole cell. We found that various 5ґ-flanking sequences could increase or decrease the transcription efficiency of a mouse 4,5SH RNA gene, but the experiments did not reveal the correlations between the structure of the 5ґ-flanking sequences and there influence on the transcription level. Apparently, there are a lot of sequences favorable for 4,5SH RNA genes transcription.
About the Authors
A. P. Koval
Учреждение Российской академии наук Институт молекулярной биологии
им. В.А. Энгельгардта РАН; Лаборатория эволюции геномов эукариот
Russian Federation
Russian Federation
D. A. Kramerov
Учреждение Российской академии наук Институт молекулярной биологии
им. В.А. Энгельгардта РАН; Лаборатория эволюции геномов эукариот
Russian Federation
Russian Federation
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Review
For citations:
Koval A.P., Kramerov D.A. DNA SEQUENCES LOCATED BEFORE 4.5SH RNA GENE CAN HAVE A GREAT IMPACT ON ITS TRANSCRIPTION BY RNA POLYMERASE III IN VITRO AND IN THE WHOLE CELL. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2011;(2):16-18. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2011-2-16-18
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