DESIGN OF  MUTANTS VARIANTS  OF  CYTOCHROME C AND  TESTING ITS  BIOLOGICAL  ACTIVITY

T. V. Ostroverkhova; R. V. Chertkova; A. N. Nekrasov; D. A. Dolgikh; M. P. Kirpichnikov

doi:10.1234/XXXX-XXXX-2011-2-25-28

DESIGN OF MUTANTS VARIANTS OF CYTOCHROME C AND TESTING ITS BIOLOGICAL ACTIVITY

T. V. Ostroverkhova, R. V. Chertkova, A. N. Nekrasov, D. A. Dolgikh, M. P. Kirpichnikov

https://doi.org/10.1234/XXXX-XXXX-2011-2-25-28

Full Text:

PDF (Rus) |

Generate QR code

Abstract

At this research work the informational structure of cytochrome c was investigated using the ANIS-method (analyse of informational structure method). The mutant genes of cytochrome c were constructed on a basis of data from ANIS-method. The mutations were turned to reduce electron-transport activity of cytochrome c at mitochondrial respiratory chain. These mutant genes were obtained and expressed at bacterial system. Recombinant proteins were purified and its bio- logical activities were tested at rat liver mitochondria.

Keywords

cytochrome c, informational structure, ANIS-method, respiratory chain, mitochondria.

About the Authors

T. V. Ostroverkhova

кафедра биоинженерии
Russian Federation

R. V. Chertkova

Институт биоорганической химии имени М.М. Шемякина и Ю.А.Овчинникова РАН, г. Москва
Russian Federation

A. N. Nekrasov

Институт биоорганической химии имени М.М. Шемякина и Ю.А.Овчинникова РАН, г. Москва
Russian Federation

D. A. Dolgikh

кафедра биоинженерии; Институт биоорганической химии имени М.М.Шемякина и Ю.А. Овчинникова РАН, г. Москва
Russian Federation

M. P. Kirpichnikov

References

1. Strekas T., Spiro T. Cytochrome c: resonance ramanspectra // Biochim. Biophys. Acta. 1972. Vol. 278(1). P. 188—192.

2. Paoli M., Liddington R., Tame J., Wilkinson A., Dodson G. Crystal structure of T state haemoglobin with oxygen bound at all four haems // J. of Mol. Biol. 1996. Vol. 256(4). P. 775—792.

3. URL:http://anis.ibch.ru/trees/ 15.10.2010.

4. Johnson D., Lardy H. Isolation of liver or kidney mitochondria // Meth. Enzymol. 1967. Vol. 10. P. 94—96.

5. Jacobs E., Sanadi D. The reversible removal of cyto- chrome c from mitochondria // J. Biol. Chem. 1960. Vol. 235. P. 531—534.

6. Пепелина Т., Черткова Р., Островерхова Т., Дол- гих Д., Кирпичников М., Гривенникова В., Виноградов А. Направленный мутагенез цитохрома с: реакции с компонентами дыхательной цепи и супероксид-радикалом // Биохимия. 2009. Т. 74. № 6. С. 768—778.

7. Некрасов А., Петровская Л., Топорова В., Крюкова Е., Родина А., Москалёва Е., Кирпичников М. Применение метода анализа информационной структуры для конструирования антагониста интерлейкина-13 // Биохимия. 2009. Т. 74. № 4. С. 493—500.

8. Nekrasov A., Zinchenko A. Structural features of the interfaces in enzyme-inhibitor complexes // J. Biomol. Struct. Dyn. 2010. Vol. 28(1). P. 85—90.

9. Nekrasov A., Zinchenko A. Hydrolases: The correlation between informational structure and the catalytic centers organization // J. Biomol. Struct. Dyn. 2008. Vol. 25(5). P. 553—562.

Review

For citations:

Ostroverkhova T.V., Chertkova R.V., Nekrasov A.N., Dolgikh D.A., Kirpichnikov M.P. DESIGN OF MUTANTS VARIANTS OF CYTOCHROME C AND TESTING ITS BIOLOGICAL ACTIVITY. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2011;(2):25-28. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2011-2-25-28

ISSN 0137-0952 (Print)

Username
Password
	Remember me
Not a user? Register with this site Forgot your password?

User

Vestnik Moskovskogo universiteta. Seriya 16. Biologiya